Onuma H, Ota M, Sugenoya A, Inoko H
Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Hum Immunol. 1994 Mar;39(3):195-201. doi: 10.1016/0198-8859(94)90260-7.
To investigate the association between HLA antigens and Graves' disease among Japanese, serologic typing and DPB1 genotyping using the PCR-RFLP method have been performed. HLA alleles of 106 patients with Graves' disease were determined, and the frequency of HLA-B46 was found to be significantly increased. Furthermore, the frequencies of HLA antigens were compared between two age groups: early-onset and late-onset patients (under and over 20 years, respectively). It was found that the frequency of DPB10501 (88.9%) was significantly increased (pc < 0.004) in the early-onset group as compared with the healthy controls (55.0%) but not in the late-onset group (60.7%). On the other hand, a significant increase of HLA-B46 was observed in the late-onset patients (pc < 0.0004). These results suggest that the genetic background of Japanese patients with early-onset Graves' disease is different from late-onset patients. Namely, the HLA-DP allele (DPB10501) and the HLA-B allele (B46) are primarily involved in the pathogenesis of early-onset and late-onset Graves' disease in Japanese, respectively.
为了研究日本人群中HLA抗原与格雷夫斯病之间的关联，我们采用聚合酶链反应-限制性片段长度多态性方法进行了血清学分型和DPB1基因分型。测定了106例格雷夫斯病患者的HLA等位基因，发现HLA - B46的频率显著增加。此外，还比较了早发型和晚发型患者（分别为20岁以下和20岁以上）两个年龄组之间的HLA抗原频率。结果发现，早发型组中DPB10501的频率（88.9%）与健康对照组（55.0%）相比显著增加（P<0.004），而晚发型组（60.7%）则无此现象。另一方面，晚发型患者中观察到HLA - B46显著增加（P<0.0004）。这些结果表明，日本早发型格雷夫斯病患者的遗传背景与晚发型患者不同。也就是说，HLA - DP等位基因（DPB10501）和HLA - B等位基因（B46）分别主要参与了日本早发型和晚发型格雷夫斯病的发病机制。
