Day R, Schafer M K, Cullinan W E, Watson S J, Chrétien M, Seidah N G
J.A. DeSève Laboratory of Biochemical, Clinical Research Institute of Montreal, Que., Canada.
Neurosci Lett. 1993 Jan 4;149(1):27-30. doi: 10.1016/0304-3940(93)90339-m.
The distribution of furin mRNA was examined in the rat central nervous system. Northern blot analysis reveals the presence of a 4.4 kb band in all brain tissues examined. In situ hybridization analysis of frozen rat brain sections using a radioactively labeled antisense cRNA probe to rat furin demonstrated moderate to low levels of expression in both neuronal and non-neuronal tissue in all areas examined. Interestingly, higher levels of furin were expressed in selective regions which include the ventricles (the choroid plexus and ependymal cells), the islands of Calleja, the hippocampus and the pineal gland. the ubiquitous localization of furin in the brain is consistent with its postulated role as a vital convertase important in the processing of proproteins negotiating the constitutive pathway of secretion. However, the higher expression of furin mRNA in distinct brain areas suggests a more active role in the processing of proproteins synthesized in these tissues.
在大鼠中枢神经系统中检测了弗林蛋白酶（furin）mRNA的分布。Northern印迹分析显示，在所检测的所有脑组织中均存在一条4.4 kb的条带。使用放射性标记的大鼠弗林蛋白酶反义cRNA探针，对冷冻的大鼠脑切片进行原位杂交分析，结果表明，在所检测的所有区域的神经元和非神经元组织中，弗林蛋白酶的表达水平均为中度至低度。有趣的是，在包括脑室（脉络丛和室管膜细胞）、卡勒哈岛、海马体和松果体在内的特定区域中，弗林蛋白酶的表达水平较高。弗林蛋白酶在大脑中的普遍定位与其作为一种重要转化酶的假定作用一致，该转化酶在处理通过组成型分泌途径的前体蛋白过程中起重要作用。然而，弗林蛋白酶mRNA在不同脑区的较高表达表明，其在这些组织中合成的前体蛋白的加工过程中发挥着更积极的作用。
