Hughes P, Dragunow M
Department of Pharmacology and Clinical Pharmacology, University of Auckland School of Medicine, New Zealand.
Neurosci Lett. 1993 Feb 5;150(1):122-6. doi: 10.1016/0304-3940(93)90122-2.
Recent studies have shown that the centrally active muscarinic agonist pilocarpine induces c-fos mRNA in rat cortex. Here we describe the localization of muscarinic receptor-induced FOS protein, within the rat central nervous system (CNS), following administration of pilocarpine (25 mg/kg). High levels of FOS induction were apparent in many forebrain structures including the primary olfactory (piriform) cortex, nucleus accumbens, amygdala, hippocampus, neocortex and supra-optic nucleus of the hypothalamus. Within the neocortex FOS induction followed a laminar distribution being highest in layers 4 and 6 with lower induction seen in layers 2 and 5. Other areas showing FOS induction included the striatum, septum, inferior colliculus, thalamus, hypothalamus and several brainstem nuclei. Both atropine (10 mg/kg) and pirenzepine (100 mg/kg) reduced FOS induction suggesting that a pirenzepine-sensitive muscarinic receptor was involved. The possible significance of muscarinic-mediated FOS induction, to cholinergic kindling and the cholinergic hypothesis of learning and memory, is discussed.
最近的研究表明，中枢活性毒蕈碱激动剂毛果芸香碱可诱导大鼠皮层中的c-fos mRNA。在此，我们描述了给予毛果芸香碱（25mg/kg）后，毒蕈碱受体诱导的FOS蛋白在大鼠中枢神经系统（CNS）中的定位。在许多前脑结构中，包括初级嗅觉（梨状）皮层、伏隔核、杏仁核、海马体、新皮层和下丘脑视上核，都明显出现了高水平的FOS诱导。在新皮层内，FOS诱导呈层状分布，第4层和第6层最高，第2层和第5层诱导较低。其他显示FOS诱导的区域包括纹状体、隔区、下丘、丘脑、下丘脑和几个脑干核。阿托品（10mg/kg）和哌仑西平（100mg/kg）都能降低FOS诱导，表明涉及一种对哌仑西平敏感的毒蕈碱受体。本文讨论了毒蕈碱介导的FOS诱导对胆碱能点燃以及学习和记忆的胆碱能假说的可能意义。
