Bucci D J, Rosen D L, Gallagher M
Neurobiology, University of North Carolina, Chapel Hill 27599, USA.
Neurobiol Aging. 1998 May-Jun;19(3):227-32. doi: 10.1016/s0197-4580(98)00051-7.
The effect of age on pilocarpine-induced expression of the immediate-early gene c-fos was examined in the hippocampus and cortex of Long-Evans rats. Rats were treated with either pilocarpine (25 mg/kg) or saline, and sacrificed 90 min. following injection. The level of c-fos mRNA and Fos-like protein expression was determined using in situ hybridization histochemistry, and immunocytochemistry, respectively. In saline-treated animals, comparable levels of c-fos mRNA and Fos-like protein were observed in the hippocampus and cortical regions of young (6 month) and aged (24-26 months) rats. The expression of Fos-like protein following pilocarpine treatment was increased, however, in frontal, retrosplenial, and cingulate cortex of aged compared to young rats. In frontal and retrosplenial cortex, the changes in Fos-like protein were accompanied by changes in c-fos mRNA expression. In contrast, no age difference was detected in the hippocampus or parietal cortex of pilocarpine-treated rats. These regionally-specific age differences in response to pilocarpine administration suggest that mechanisms localized to those areas play an important role in determining the response to cholinergic stimulation mediated through post-synaptic muscarinic receptors.
在Long-Evans大鼠的海马体和皮层中，研究了年龄对毛果芸香碱诱导的即刻早期基因c-fos表达的影响。给大鼠注射毛果芸香碱（25 mg/kg）或生理盐水，注射90分钟后处死。分别使用原位杂交组织化学和免疫细胞化学方法测定c-fos mRNA水平和Fos样蛋白表达。在生理盐水处理的动物中，在年轻（6个月）和老年（24 - 26个月）大鼠的海马体和皮层区域观察到相当水平的c-fos mRNA和Fos样蛋白。然而，与年轻大鼠相比，毛果芸香碱处理后，老年大鼠额叶、脾后叶和扣带回皮层中Fos样蛋白的表达增加。在额叶和脾后叶皮层中，Fos样蛋白的变化伴随着c-fos mRNA表达的变化。相比之下，在毛果芸香碱处理的大鼠的海马体或顶叶皮层中未检测到年龄差异。这些对毛果芸香碱给药的区域特异性年龄差异表明，定位于这些区域的机制在决定对通过突触后毒蕈碱受体介导的胆碱能刺激的反应中起重要作用。
