Sobolewski B, Mackenstedt U, Mehlhorn H
Lehrstuhl für Spezielle Zoologie und Parasitologie, Ruhr-Universität Bochum, Germany.
Parasitol Res. 1993;79(1):33-41. doi: 10.1007/BF00931215.
A new method for the isolation of intraerythrocytic stages of Plasmodium berghei and Babesia divergens from red blood cells is described. The technique is based on hydrodynamic forces occurring in a flow channel containing a turbulent liquid current, which are capable of rupturing infected erythrocytes and removing their plasma membrane from the parasites' surface. The temperature and the concentration of cells were revealed as factors influencing the hydrodynamic forces. About 90% of the intact and apparently infectious parasites of both species were isolated from the lysed erythrocytes.
描述了一种从红细胞中分离伯氏疟原虫和分歧巴贝斯虫红细胞内期的新方法。该技术基于在含有湍流液体流的流动通道中产生的流体动力，这种流体动力能够使受感染的红细胞破裂，并从寄生虫表面去除其质膜。温度和细胞浓度被揭示为影响流体动力的因素。两种物种中约90%完整且明显具有感染性的寄生虫从裂解的红细胞中分离出来。
