Koi M, Johnson L A, Kalikin L M, Little P F, Nakamura Y, Feinberg A P
Howard Hughes Medical Institute, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109.
Science. 1993 Apr 16;260(5106):361-4. doi: 10.1126/science.8469989.
A fundamental problem in the identification and isolation of tumor suppressor and other growth-inhibiting genes is the loss of power of genetic complementation at the subchromosomal level. A direct genetic strategy was developed to isolate subchromosomal transferable fragments (STFs) from any chromosome, each containing a selectable marker within the human DNA, that could be transferred to any mammalian cell. As a test of the method, several overlapping STFs from 11p15 were shown to cause in vitro growth arrest of rhabdomyosarcoma cells. This activity mapped between the beta-globin and insulin genes.
在肿瘤抑制基因和其他生长抑制基因的鉴定与分离过程中，一个基本问题是亚染色体水平上遗传互补能力的丧失。我们开发了一种直接的遗传策略，用于从任何染色体中分离亚染色体可转移片段（STF），每个片段在人类DNA中都包含一个选择标记，并且可以转移到任何哺乳动物细胞中。作为该方法的一个测试，来自11p15的几个重叠STF被证明可导致横纹肌肉瘤细胞在体外生长停滞。这种活性定位于β-珠蛋白基因和胰岛素基因之间。
