Haugen O A, Aasmundstad T, Johannesen E, Ytterhus B, Nervik I
Avdeling for patologi, Regionsykehuset i Trondheim.
Tidsskr Nor Laegeforen. 1993 Mar 20;113(8):944-6.
By application of a monoclonal antibody (Abbott ER-ICA) oestrogen receptors can now be demonstrated and semiquantitated on paraffin sections after fixation in formalin. In a prospective series of 22 breast carcinomas we found that 82% (18/22) were oestrogen receptor positive. The frequency was significantly lower in archival tissue comprising 27 invasive carcinomas (56%) and in 63 intraductal carcinomas (54%). Even lower frequencies were found in recurrences and metastases and none (0/10) of the metastases from tumours of unknown origin were oestrogen receptor positive. It is likely that prolonged fixation in formalin destroys the receptor protein. Immunocytochemical assessment of oestrogen receptor may be the only alternative when dealing with small breast tumours (< 1 cm), in cases of suspected recurrences and in cases of unknown receptor status.
通过应用单克隆抗体（雅培ER-ICA），现在可以在福尔马林固定后的石蜡切片上显示雌激素受体并进行半定量分析。在一项对22例乳腺癌的前瞻性研究中，我们发现82%（18/22）的病例雌激素受体呈阳性。在包含27例浸润性癌（56%）的存档组织和63例导管内癌（54%）中，这一频率显著降低。在复发和转移病例中发现的频率更低，且来源不明肿瘤的转移灶中无一例（0/10）雌激素受体呈阳性。很可能是福尔马林的长时间固定破坏了受体蛋白。在处理小乳腺肿瘤（<1 cm）、疑似复发病例以及受体状态不明的病例时，雌激素受体的免疫细胞化学评估可能是唯一的选择。
