Johnson E K, Ownby C L
Department of Physiological Sciences, Oklahoma State University, Stillwater 74078.
Toxicon. 1993 Mar;31(3):243-55. doi: 10.1016/0041-0101(93)90143-7.
A myotoxin was isolated from the venom of the broad-banded copperhead (Agkistrodon contortrix laticinctus) by HPLC using anion and cation exchange chromatography. The toxin has a mol. wt of approximately 14,000 and has a pI greater than 9. It does not have phospholipase A activity, but does induce myonecrosis of skeletal muscle cells characterized by a hypercontraction of myofilaments. Electron microscopic analysis showed that the myotoxin appears to disrupt the sarcolemma of skeletal muscle cells. ACL myotoxin is very similar in mol. wt, amino acid composition, and myotoxic activity to myotoxins isolated from the venoms of Bothrops asper and Bothrops nummifer from Central America, suggesting that homologs of this toxin may be found in other crotaline snake venoms.
通过使用阴离子和阳离子交换色谱的高效液相色谱法，从宽带铜头蝮蛇（Agkistrodon contortrix laticinctus）的毒液中分离出一种肌毒素。该毒素的分子量约为14,000，其等电点大于9。它不具有磷脂酶A活性，但会诱导骨骼肌细胞的肌坏死，其特征是肌丝过度收缩。电子显微镜分析表明，该肌毒素似乎会破坏骨骼肌细胞的肌膜。ACL肌毒素在分子量、氨基酸组成和肌毒性活性方面与从中美洲的矛头蝮蛇（Bothrops asper）和中美矛头蝮蛇（Bothrops nummifer）毒液中分离出的肌毒素非常相似，这表明这种毒素的同源物可能存在于其他蝰蛇科蛇毒中。
