Igarashi Y, Kawamata S
Department of Otolaryngology, Toyama Medical and Pharmaceutical University, Japan.
Acta Otolaryngol Suppl. 1993;504:140-2. doi: 10.3109/00016489309128141.
The tympanic membrane of the guinea pig was studied by electron microscopy. The pars tensa was composed of three layers, i.e., the outer epidermis, the middle lamina propria and the inner mucosal membrane. The pars flaccida was negligible in size. The fibers in the lamina propria of the tympanic membrane were not clearly observable by conventional electron microscopy. With the silver methenamine method the fibers were more intensely stained than the epithelial cells and could thus be distinctly demonstrated by this method. In the inferior larger portion of the pars tensa, the lamina propria contained the outer radial and the inner circular fiber bundles. The radial fiber bundles were thicker and more densely packed than the circular ones. This fact indicates that the radial fibers form the major skeleton of this portion. The superior smaller portion of the pars tensa contained several layers of irregularly arranged fiber bundles in the lamina propria and was considerably thicker than the inferior larger portion.
通过电子显微镜对豚鼠的鼓膜进行了研究。紧张部由三层组成,即外层的表皮、中间的固有层和内层的黏膜。松弛部尺寸可忽略不计。常规电子显微镜下无法清晰观察到鼓膜固有层中的纤维。用六胺银法,纤维比上皮细胞染色更深,因此可通过该方法清楚地显示出来。在紧张部下方较大部分,固有层包含外层的放射状纤维束和内层的环状纤维束。放射状纤维束比环状纤维束更粗且排列更密集。这一事实表明放射状纤维构成了该部分的主要骨架。紧张部上方较小部分的固有层包含几层不规则排列的纤维束,且比下方较大部分厚得多。