使用掺杂银细颗粒的聚乙烯醇薄膜对碳酸根离子、亚硫酸根离子和核酸碱基进行表面增强拉曼光谱检测。

Surface-enhanced Raman spectroscopic detection of CO2-(3), SO2-(3), and nucleic acid bases using polyvinyl alcohol film doped with Ag fine particles.

作者信息

Kurokawa Y, Imai Y, Sasaki Y, Maeda T

机构信息

Department of Science Engineering, Faculty of Engineering, Tohoku University, Sendai, Japan.

出版信息

Anal Biochem. 1993 Mar;209(2):247-50. doi: 10.1006/abio.1993.1115.

DOI:10.1006/abio.1993.1115

PMID:8470796

Abstract

Surface-enhanced Raman scattering (SERS)-active polyvinyl alcohol films doped with Ag particles have been prepared by a counterdiffusion method. SERS spectra of CO2-(3), SO2-(3), and DNA bases have been observed by using the films. The films containing aggregated Ag particles are useful to observe SERS spectra of adsorbates. DNA bases (cytosine, uracil, thymine, guanine, and adenine) can be detected at a level of 10(-8)-10(-9) M by this method.

摘要

通过反扩散法制备了掺杂银颗粒的具有表面增强拉曼散射（SERS）活性的聚乙烯醇薄膜。利用这些薄膜观察了CO₂⁻³、SO₂⁻³和DNA碱基的SERS光谱。含有聚集银颗粒的薄膜对于观察吸附质的SERS光谱很有用。通过这种方法可以检测到10⁻⁸ - 10⁻⁹ M水平的DNA碱基（胞嘧啶、尿嘧啶、胸腺嘧啶、鸟嘌呤和腺嘌呤）。