鸡膜联蛋白V在2.25埃分辨率下的结构

Structure of chicken annexin V at 2.25-A resolution.

作者信息

Bewley M C, Boustead C M, Walker J H, Waller D A, Huber R

机构信息

Department of Biochemistry and Molecular Biology, University of Leeds, England.

出版信息

Biochemistry. 1993 Apr 20;32(15):3923-9. doi: 10.1021/bi00066a011.

DOI:10.1021/bi00066a011

PMID:8471604

Abstract

The crystal structure of chicken annexin V has been solved by molecular replacement and refined at 2.25 A. The final R factor is 19.7% with good geometry. The chicken annexin V structure is very similar to the human annexin V structure, with four similar domains each containing five helices. The structure includes three calcium ions in domains I, II, and IV, each bound by the characteristic K-G-X-G-T-(38 residues)-D/E motif. In view of the structural similarity between human and chicken annexin V, we suggest that they have a common vital function which developed early in evolutionary history.

摘要

鸡膜联蛋白V的晶体结构已通过分子置换法解析，并在2.25埃分辨率下进行了精修。最终的R因子为19.7%，几何结构良好。鸡膜联蛋白V的结构与人类膜联蛋白V的结构非常相似，有四个相似的结构域，每个结构域包含五个螺旋。该结构在结构域I、II和IV中包含三个钙离子，每个钙离子都由特征性的K-G-X-G-T-（38个残基）-D/E基序结合。鉴于人类和鸡膜联蛋白V之间的结构相似性，我们认为它们具有共同的重要功能，这一功能在进化史上很早就已形成。