Tobal K, Deuble K, McCartney A, Lightman S
Department of Clinical Science, Institute of Ophthalmology, London, UK.
Melanoma Res. 1993 Feb;3(1):63-5. doi: 10.1097/00008390-199304000-00009.
An immunohistochemical double staining technique was used to examine the characteristics of cellular infiltration in choroidal melanoma. Seven of 16 melanomas examined demonstrated high levels of cellular infiltration, mainly with T-cells and macrophages, and little infiltration with B-cells and NK cells. The majority of T-cells were of the CD8+ type and were activated, as shown by the expression of histocompatibility antigens, HLA-DR and IL2-R. Most of the infiltrating macrophages also expressed HLA-DR antigen. We also detected malignant melanocytes expressing the HLA-DR antigen. This technique could be used to study in detail cellular infiltration in a large number of archival choroidal melanomas with known clinical history, which would enable detection of markers that correlate with the prognosis of the disease.
采用免疫组织化学双重染色技术检测脉络膜黑色素瘤的细胞浸润特征。在检测的16例黑色素瘤中，有7例显示出高水平的细胞浸润，主要为T细胞和巨噬细胞浸润，B细胞和NK细胞浸润较少。大多数T细胞为CD8+型且被激活，这通过组织相容性抗原、HLA-DR和IL2-R的表达得以体现。大多数浸润性巨噬细胞也表达HLA-DR抗原。我们还检测到表达HLA-DR抗原的恶性黑色素细胞。该技术可用于详细研究大量具有已知临床病史的存档脉络膜黑色素瘤中的细胞浸润情况，这将有助于检测与疾病预后相关的标志物。
