Ohta K, Norose K, Ishii K
Department of Ophthalmology, Shinshu University School of Medicine, Nagano-ken, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Nov;99(11):1277-82.
We performed immunohistochemistry on tumor infiltrating lymphocytes (TILs) in a 78-year-old man with choroidal malignant melanoma. Cell suspensions of TILs from fresh specimens and peripheral blood lymphocytes (PBLs) were stained with anti-CD3, anti-CD4, anti-CD8, anti-CD29, anti-CD45RA, and anti-human leukocyte antigen (HLA)-DR monoclonal antibodies and analyzed using three-color flow cytometry. In light microscopy, the number of infiltrating lymphocytes around the tumor was very small. Immunohistochemically, T lymphocytes were more numerous than B lymphocytes. Flow cytometric analysis showed that CD8+ cells were more numerous than CD4+ cells in CD3+ cells in TILs, and most of these cells also expressed HLA-DR antigen. CD29+ (memory) cells were increased and CD45RA+ (naive) cells were decreased in CD4+ cells in TILs as compared with PBLs. We concluded that the increase in the percentage of activated memory T lymphocytes and the decrease of naive T lymphocytes may reflect a localized antigen-specific immunological response in choroidal malignant melanoma.
我们对一名78岁脉络膜恶性黑色素瘤男性患者的肿瘤浸润淋巴细胞（TILs）进行了免疫组织化学检测。用抗CD3、抗CD4、抗CD8、抗CD29、抗CD45RA和抗人类白细胞抗原（HLA）-DR单克隆抗体对新鲜标本中的TILs细胞悬液和外周血淋巴细胞（PBLs）进行染色，并使用三色流式细胞术进行分析。在光学显微镜下，肿瘤周围浸润淋巴细胞的数量非常少。免疫组织化学显示，T淋巴细胞比B淋巴细胞更多。流式细胞术分析表明，TILs中CD3+细胞内CD8+细胞比CD4+细胞更多，并且这些细胞中的大多数也表达HLA-DR抗原。与PBLs相比，TILs中CD4+细胞内CD29+（记忆）细胞增加而CD45RA+（幼稚）细胞减少。我们得出结论，活化记忆T淋巴细胞百分比的增加和幼稚T淋巴细胞的减少可能反映了脉络膜恶性黑色素瘤中的局部抗原特异性免疫反应。
