Devdariani Z L, Verenkov M S, Feodorova V A, Solodovnicov N S, Belov L G
Russia State Antiplague Research Institute Microbe, Saratov.
FEMS Immunol Med Microbiol. 1993 Jan;6(1):31-5. doi: 10.1111/j.1574-695X.1993.tb00300.x.
The efficiency of serological identification of Yersinia pestis strains which contain different plasmids was assessed with polyclonal and monoclonal immunoglobulin preparations in the direct fluorescent antibody method. Plague polyclonal luminescent immunoglobulins recognize only those Y. pestis strains which contain pPst, pFra plasmids or both. Anticapsular plague monoclonal antibodies interact only with capsule-forming plague agent strains (pFra+) grown at 37 degrees C. With plague monoclonal lipopolysaccharide antibodies one can identify all Y. pestis strains irrespective of their plasmid content and cultivation temperature. However, these antibodies cross-react with Yersinia pseudotuberculosis bacteria in 60% of cases. The problem of laboratory diagnosis of the plague organism, whatever its plasmid profile, can be solved through the development of a test kit involving two preparations such as plague lipopolysaccharide monoclonal luminescent antibodies and pseudotuberculosis specific luminescent adsorbed immunoglobulins.
采用多克隆和单克隆免疫球蛋白制剂，通过直接荧光抗体法评估了含有不同质粒的鼠疫耶尔森菌菌株的血清学鉴定效率。鼠疫多克隆发光免疫球蛋白仅识别那些含有pPst、pFra质粒或两者皆有的鼠疫耶尔森菌菌株。抗荚膜鼠疫单克隆抗体仅与在37℃下生长的形成荚膜的鼠疫病原体菌株（pFra+）相互作用。使用鼠疫单克隆脂多糖抗体，可以识别所有鼠疫耶尔森菌菌株，无论其质粒含量和培养温度如何。然而，这些抗体在60%的情况下与假结核耶尔森菌发生交叉反应。无论鼠疫病原体的质粒谱如何，通过开发一种包含两种制剂（如鼠疫脂多糖单克隆发光抗体和假结核特异性发光吸附免疫球蛋白）的检测试剂盒，都可以解决鼠疫病原体的实验室诊断问题。
