Lou S C, Patel C, Ching S, Gordon J
Abbott Diagnostics Division, Abbott Laboratories, North Chicago, IL 60064-3500.
Clin Chem. 1993 Apr;39(4):619-24.
Numerous studies have associated high concentrations of lipoprotein(a) [Lp(a)] with atherosclerosis. We developed a rapid, one-step competitive immunochromatographic assay to measure Lp(a) in plasma. The assay is performed on a nitrocellulose membrane strip and the result is determined by a visual readout of rust-colored colloidal selenium. The assay is based on the principle that Lp(a) in the sample will compete with Lp(a)-coated colloidal selenium for binding to the anti-Lp(a) monoclonal antibody immobilized on the assay strip in the format of four ladder bars. The number of capture bars that appear as a result of the formation of colloidal selenium color is proportional to the concentration of the Lp(a) protein in the samples. The strip assay semiquantitatively measures Lp(a) concentrations ranging from 0 to 180 mg/L of Lp(a) protein in serum, plasma, or fingerstick whole-blood samples. This assay appears very useful for quick identification of individuals with above-normal concentrations of plasma Lp(a) protein (> 70 mg/L), and has potential for monitoring a patient's response to treatment with Lp(a)-lowering drugs.
大量研究已将高浓度脂蛋白(a)[Lp(a)]与动脉粥样硬化联系起来。我们开发了一种快速的一步竞争性免疫层析测定法来测量血浆中的Lp(a)。该测定在硝酸纤维素膜条上进行，结果通过肉眼读取锈色胶体硒来确定。该测定基于这样的原理：样品中的Lp(a)会与包被Lp(a)的胶体硒竞争，以结合固定在测定条上呈四个梯状条形式的抗Lp(a)单克隆抗体。由于形成胶体硒颜色而出现的捕获条数量与样品中Lp(a)蛋白的浓度成正比。该条带测定法可半定量测量血清、血浆或指尖全血样品中Lp(a)蛋白浓度范围为0至180mg/L的Lp(a)。该测定法对于快速识别血浆Lp(a)蛋白浓度高于正常水平（>70mg/L）的个体似乎非常有用，并且具有监测患者对降低Lp(a)药物治疗反应的潜力。
