一种用于快速检测水中溴苯腈的免疫层析测定法的开发。
Development of an immunochromatographic assay for the rapid detection of bromoxynil in water.
作者信息
Zhu Jiang, Chen Wenchao, Lu Yitong, Cheng Guohua
机构信息
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
出版信息
Environ Pollut. 2008 Nov;156(1):136-42. doi: 10.1016/j.envpol.2007.12.020. Epub 2008 Feb 6.
A rapid immunochromatographic one-step strip test was developed to specifically determine bromoxynil in surface and drinking water by competitive inhibition with the nano colloidal gold-conjugated monoclonal antibody (mAb). Bromoxynil standard samples of 0.01-10 mg L(-1) in water were tested by this method and the visual limit was 0.06 mg L(-1). The assay only required 5 min and one-step by dispensing a drop of sample solution onto a strip. Parallel analysis of water samples with bromoxynil showed comparable results from one-step strip test and ELISA. Therefore, the one-step strip test is very useful as a screening method for qualitative detection of bromoxynil in water.
开发了一种快速免疫层析一步法试纸条检测方法，通过与纳米胶体金偶联单克隆抗体（mAb）竞争抑制，特异性测定地表水和饮用水中的溴苯腈。用该方法检测了水中0.01-10 mg L(-1)的溴苯腈标准样品，目视检测限为0.06 mg L(-1)。该检测只需5分钟，通过将一滴样品溶液滴到试纸上即可一步完成。对含有溴苯腈的水样进行平行分析表明，一步法试纸条检测和酶联免疫吸附测定（ELISA）结果相当。因此，一步法试纸条检测作为水中溴苯腈定性检测的筛查方法非常有用。
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