用于测定肝脏中氧化型和还原型谷胱甘肽的自动分光光度法。
Automated spectrophotometric method for determining oxidized and reduced glutathione in liver.
作者信息
Teare J P, Punchard N A, Powell J J, Lumb P J, Mitchell W D, Thompson R P
机构信息
Gastrointestional Laboratory, Rayne Institute, London, UK.
出版信息
Clin Chem. 1993 Apr;39(4):686-9.
An enzymatic recycling method has been applied to the measurement of total and oxidized glutathione with a centrifugal analyzer. When the reduced form of glutathione (GSH) was masked with 2-vinylpyridine to measure the oxidized glutathione (GSSG), the time to ensure full derivatization was three times longer than has been reported. The method is quick, simple, accurate, and precise (1.27% for GSH, 3.3% for GSSG intraassay CV; 2.15% for GSH, 5% for GSSG interassay CV), and the automation allows large numbers of samples to be conveniently assayed.
一种酶循环法已应用于用离心分析仪测定总谷胱甘肽和氧化型谷胱甘肽。在用2-乙烯基吡啶掩蔽还原型谷胱甘肽（GSH）以测定氧化型谷胱甘肽（GSSG）时，确保完全衍生化的时间比已报道的长三倍。该方法快速、简单、准确且精密（GSH批内变异系数为1.27%，GSSG为3.3%；GSH批间变异系数为2.15%，GSSG为5%），并且自动化操作使得大量样品能够方便地进行检测。
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