Kazwala R R, Jiwa S F, Nkya A E
Department of Veterinary Medicine and Public Health, Sokoine University of Agriculture, Morogoro, Tanzania.
Epidemiol Infect. 1993 Apr;110(2):273-8. doi: 10.1017/s0950268800068205.
A total of 255 samples of droppings collected from a total of 22 different poultry units were examined for the presence of thermophilic campylobacters and the isolates biotyped using Skirrow's protocol. The organisms were isolated from 90 (35.3%) of all samples. Among the 22 units investigated, 13 (59%) were found to have unsatisfactory management systems, while 7 (32%) and 2 (9%) were found to have unsatisfactory and good systems respectively. Significantly large numbers of isolations, 68 of 147 (46.2%), were made from samples collected from poultry units with poor management (P < 0.005), compared with 19 out of 84 (22.6%) samples which were collected from satisfactory units and 3 out of 24 (12.5%) samples collected from units exercising particularly good management. Nineteen of 72 (26.4%) samples collected from broilers, 32 out of 132 (24.2%) samples collected from layers and 39 out of 51 (76.49%) samples collected from indigenous free range poultry were positive for campylobacters. Among the 90 strains isolated from various units, 64 (70.1%) were Campylobacter jejuni, 25 (27.7%) were C. coli, and only 1 (2.2%) was C. laridis.
共从22个不同的家禽养殖单元收集了255份粪便样本，检测其中嗜热弯曲杆菌的存在情况，并按照斯基罗方法对分离菌株进行生物分型。在所有样本中，90份（35.3%）分离出了该微生物。在所调查的22个养殖单元中，13个（59%）被发现管理系统不佳，7个（32%）和2个（9%）分别被发现管理系统不满意和良好。与从管理良好的单元收集的24份样本中的3份（12.5%）以及从管理令人满意的单元收集的84份样本中的19份（22.6%）相比，从管理不善的家禽养殖单元收集的样本中分离出的数量显著更多，147份中有68份（46.2%）（P<0.005）。从肉鸡收集的72份样本中有19份（26.4%）、从蛋鸡收集的132份样本中有32份（24.2%）以及从本地散养家禽收集的51份样本中有39份（76.49%）弯曲杆菌呈阳性。在从各个单元分离出的90株菌株中，64株（70.1%）为空肠弯曲杆菌，25株（27.7%）为大肠弯曲杆菌，只有1株（2.2%）为拉氏弯曲杆菌。
