High-pressure liquid chromatographic (HPLC) determination of tolmetin and its major metabolite in plasma.

A high-pressure liquid chromatographic assay was developed for the quantitative determination of tolmetin and its major metabolite in plasma. Determination of tolmetin was compared to a previously reported spectro-photometric assay method. The standard curves were evaluated statistically and daily standard curves were preferred over a pooled standard curve. The method was applied to samples of one subject's plasma containing both tolmetin and its major metabolite.