采用高压液相色谱法测定血浆中佐美酸的含量。

Determination of zomepirac in plasma by high-pressure liquid chromatography.

作者信息

Ng K T, Snyderman T

出版信息

J Chromatogr. 1979 Oct 19;178(1):241-7. doi: 10.1016/s0021-9673(00)89712-5.

Abstract

A sensitive, specific and precise high-pressure liquid-chromatographic method for the determination of the analgesic agent, zomepirac, in plasma samples is described. The lowest concentration of zomepirac that can be measured accurately and precisely (coefficient of variation less than 20%) in a 2 ml plasma sample is 10 ng/ml. The standard curve is linear in the concentration range of 10 to 5000 ng/ml. To date, this procedure has been employed successfully in analysing over 10,000 clinical plasma samples.

摘要

本文描述了一种灵敏、特异且精确的高压液相色谱法，用于测定血浆样本中的镇痛剂佐美酸。在2ml血浆样本中能够准确且精确测量（变异系数小于20%）的佐美酸最低浓度为10ng/ml。标准曲线在10至5000ng/ml的浓度范围内呈线性。迄今为止，该方法已成功应用于分析超过10000份临床血浆样本。