Fujimoto K, Fukagawa K, Sakata T, Tso P
Department of Physiology, Lousiana State University Medical Center, Shreveport 71130.
J Clin Invest. 1993 Apr;91(4):1830-3. doi: 10.1172/JCI116395.
The aim of this experiment was to investigate whether the anorectic effect of apolipoprotein A-IV (apo A-IV) after lipid feeding is mediated via the central nervous system. Infusion of 0.5 micrograms of apo A-IV into the third ventricle failed to suppress food intake. Higher doses (1 micrograms or higher) of apo A-IV infused into the third ventricle inhibited food intake in a dose-dependent manner. In contrast, when apo A-I was infused into the third ventricle it had no effect on food intake. To further test the hypothesis that apo A-IV is an important factor controlling food intake, we administered goat anti-rat apo A-IV serum into the third ventricle of rats that were allowed food and water and lib. In all rats tested, this treatment resulted in enhanced food intake. In contrast, infusion of goat anti-rat apo A-IV serum failed to elicit such a response. Lastly, we determined the apo A-IV concentration in plasma and cerebrospinal fluid before and during active lipid absorption. Apo A-IV concentration in cerebrospinal fluid was about 1/20 that of plasma. Both serum and cerebrospinal fluid apo A-IV increased markedly as a result of feeding of lipid. In conclusion, we propose that apo A-IV may act centrally to control food intake.
本实验的目的是研究脂质喂养后载脂蛋白A-IV（apo A-IV）的厌食作用是否通过中枢神经系统介导。向第三脑室注入0.5微克的apo A-IV未能抑制食物摄入。向第三脑室注入更高剂量（1微克或更高）的apo A-IV以剂量依赖的方式抑制食物摄入。相比之下，当向第三脑室注入apo A-I时，对食物摄入没有影响。为了进一步检验apo A-IV是控制食物摄入的重要因素这一假设，我们将山羊抗大鼠apo A-IV血清注入可自由获取食物和水的大鼠的第三脑室。在所有测试的大鼠中，这种处理导致食物摄入量增加。相比之下，注入山羊抗大鼠apo A-IV血清未能引发这种反应。最后，我们测定了活跃脂质吸收前后血浆和脑脊液中apo A-IV的浓度。脑脊液中apo A-IV的浓度约为血浆浓度的1/20。由于脂质喂养，血清和脑脊液中的apo A-IV均显著增加。总之，我们认为apo A-IV可能在中枢发挥作用以控制食物摄入。
