Karunaratne K M, Plapp F W
Department of Entomology, Texas A&M University, College Station 77843-2475.
J Econ Entomol. 1993 Apr;86(2):258-64. doi: 10.1093/jee/86.2.258.
The biochemistry and genetics of thiodicarb resistance were studied in the house fly, Musca domestica L. Bioassays with several strains revealed that a thiodicarb-resistant strain was also resistant to carbaryl and to several organophosphates. Bioassays with a thiodicarb-synergist combination showed that resistance was only partially blocked by use of a synergist, suggesting the presence of target site resistance. The inheritance of resistance to thiodicarb was studied through crosses of the resistant parent to a multimutant, susceptible strain. Bioassays of backcrosses and the F2 generation showed that the major gene(s) conferring resistance to thiodicarb were on chromosome II. Cholinesterase assays showed that the enzyme of the resistant strain was several times more resistant to inhibition by carbamates than the enzyme of a susceptible strain or a strain with only metabolic resistance to insecticides. The data indicate that resistance to thiodicarb in the house fly was mainly because of target site changes. In addition, metabolism may contribute to resistance.
对家蝇（Musca domestica L.）中抗硫双威的生物化学和遗传学进行了研究。对多个品系的生物测定表明，一个抗硫双威的品系也对西维因和几种有机磷酸酯具有抗性。用硫双威增效剂组合进行的生物测定表明，使用增效剂仅部分阻断了抗性，这表明存在靶标位点抗性。通过将抗性亲本与一个多突变易感品系杂交，研究了对硫双威抗性的遗传。回交和F2代的生物测定表明，赋予对硫双威抗性的主要基因位于二号染色体上。胆碱酯酶测定表明，抗性品系的酶对氨基甲酸酯抑制的抗性比易感品系或仅对杀虫剂具有代谢抗性的品系的酶高几倍。数据表明，家蝇对硫双威的抗性主要是由于靶标位点的变化。此外，代谢可能也有助于产生抗性。
