Hamaoka T, Mori Y, Terakado N, Nakamura S
Touhoku Branch Laboratory, National Institute of Animal Health, Aomori, Japan.
J Gen Microbiol. 1993 Mar;139(3):617-22. doi: 10.1099/00221287-139-3-617.
The EDTA-soluble antigens were prepared from whole cells of six strains of Clostridium chauvoei and five strains of C. septicum and were compared by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot analysis. SDS-PAGE profiles of the 11 strains were nearly identical, although there were slight variations in molecular mass in adjacent bands. In immunoblot analysis with two antisera against C. chauvoei and three against C. septicum, the antigens of all strains tested reacted with all five antisera and there were no differences in reactivities to the same antiserum between homologous and heterologous antigens. In an immunoblot reacted with a single antiserum, band patterns of 10 of the 11 strains were quite similar. After cross-absorption, antisera to both species lost most of their reactivities not only to heterologous antigens but also to homologous antigens. These results indicate that the two species share many common antigens and that there is a marked similarity in the antigenic properties of EDTA-soluble material.
从6株产气荚膜梭菌和5株败血梭菌的全细胞中制备乙二胺四乙酸（EDTA）可溶性抗原，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹分析进行比较。11株菌的SDS-PAGE图谱几乎相同，尽管相邻条带的分子量存在细微差异。在用两种抗产气荚膜梭菌抗血清和三种抗败血梭菌抗血清进行的免疫印迹分析中，所有测试菌株的抗原均与所有五种抗血清发生反应，同源抗原和异源抗原对同一种抗血清的反应性没有差异。在与单一抗血清反应的免疫印迹中，11株菌中有10株的条带模式非常相似。交叉吸收后，两种菌的抗血清不仅对异源抗原，而且对同源抗原的大部分反应性都丧失了。这些结果表明，这两种菌共享许多共同抗原，并且EDTA可溶性物质的抗原特性存在明显相似性。
