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在非洲爪蟾中，一种受发育调控的信使核糖核酸的产物在结构和功能上与酿酒酵母中一种参与翻译保真度的蛋白质同源。

In Xenopus laevis, the product of a developmentally regulated mRNA is structurally and functionally homologous to a Saccharomyces cerevisiae protein involved in translation fidelity.

作者信息

Tassan J P, Le Guellec K, Kress M, Faure M, Camonis J, Jacquet M, Philippe M

机构信息

Département de Biologie et Génétique du Développement, CNRS URA 256, Université de Rennes I, France.

出版信息

Mol Cell Biol. 1993 May;13(5):2815-21. doi: 10.1128/mcb.13.5.2815-2821.1993.

DOI:10.1128/mcb.13.5.2815-2821.1993
PMID:8474443
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC359665/
Abstract

We have performed a differential screen of a Xenopus egg cDNA library and selected two clones (Cl1 and Cl2) corresponding to mRNA which are specifically adenylated and recruited into polysomes after fertilization. Sequence analysis of Cl1 reveals that the corresponding protein is 67.5% identical (83% similar) to the product of the Saccharomyces cerevisiae SUP45 (also called SUP1 or SAL4) gene. This gene, when mutated, is an omnipotent suppressor of nonsense codons. When expressed in a sup45 mutant, the Xenopus Cl1 cDNA was able to suppress sup45-related phenotypes, showing that the structural homology reflects a functional homology. Our discovery of a structural and functional homolog in Xenopus cells implies that the function of SUP45 is not restricted to lower eukaryotes and that the SUP45 protein may perform a crucial cellular function in higher eukaryotes.

摘要

我们对非洲爪蟾卵cDNA文库进行了差异筛选，挑选出两个与受精后经特异性腺苷酸化并进入多核糖体的mRNA相对应的克隆（Cl1和Cl2）。对Cl1的序列分析显示，相应蛋白质与酿酒酵母SUP45（也称为SUP1或SAL4）基因的产物有67.5%的同一性（83%的相似性）。该基因发生突变时，是无义密码子的全能抑制子。当在sup45突变体中表达时，非洲爪蟾Cl1 cDNA能够抑制与sup45相关的表型，这表明结构同源性反映了功能同源性。我们在非洲爪蟾细胞中发现结构和功能同源物意味着SUP45的功能并不局限于低等真核生物，并且SUP45蛋白可能在高等真核生物中执行关键的细胞功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/6c2e51ae4866/molcellb00017-0194-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/e7fc51b8238d/molcellb00017-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/3ce9b6841d16/molcellb00017-0193-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/c938828400bc/molcellb00017-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/6c2e51ae4866/molcellb00017-0194-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/e7fc51b8238d/molcellb00017-0193-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/3ce9b6841d16/molcellb00017-0193-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/c938828400bc/molcellb00017-0194-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cac/359665/6c2e51ae4866/molcellb00017-0194-b.jpg

相似文献

1
In Xenopus laevis, the product of a developmentally regulated mRNA is structurally and functionally homologous to a Saccharomyces cerevisiae protein involved in translation fidelity.在非洲爪蟾中，一种受发育调控的信使核糖核酸的产物在结构和功能上与酿酒酵母中一种参与翻译保真度的蛋白质同源。
Mol Cell Biol. 1993 May;13(5):2815-21. doi: 10.1128/mcb.13.5.2815-2821.1993.
2
Reversions to respiratory competence of omnipotent sup45 suppressor mutants may be caused by secondary sup45 mutations.全能性sup45抑制突变体恢复呼吸能力可能是由继发性sup45突变引起的。
Curr Genet. 1995 Feb;27(3):195-200. doi: 10.1007/BF00326148.
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The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae.SUP45（eRF1）基因和SUP35基因的产物相互作用，介导酿酒酵母中的翻译终止。
EMBO J. 1995 Sep 1;14(17):4365-73. doi: 10.1002/j.1460-2075.1995.tb00111.x.
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A conditional-lethal translation termination defect in a sup45 mutant of the yeast Saccharomyces cerevisiae.酿酒酵母sup45突变体中的条件致死性翻译终止缺陷。
Eur J Biochem. 1997 May 1;245(3):557-63. doi: 10.1111/j.1432-1033.1997.00557.x.
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A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor.一个具有多肽链释放因子特性的高度保守的真核蛋白家族。
Nature. 1994 Dec 15;372(6507):701-3. doi: 10.1038/372701a0.
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Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2.通过差异筛选非洲爪蟾cDNA克隆一种编码与cdc2高度同源蛋白质的基因。
Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):1039-43. doi: 10.1073/pnas.88.3.1039.
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Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae.NMD的失活增加了酿酒酵母中sup45无义突变体的存活率。
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The allosuppressor gene SAL4 encodes a protein important for maintaining translational fidelity in Saccharomyces cerevisiae.同种抑制基因SAL4编码一种对维持酿酒酵母翻译保真度很重要的蛋白质。
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Isolation of the SUP45 omnipotent suppressor gene of Saccharomyces cerevisiae and characterization of its gene product.酿酒酵母SUP45全能抑制基因的分离及其基因产物的特性分析。
Mol Cell Biol. 1985 Apr;5(4):816-22. doi: 10.1128/mcb.5.4.816-822.1985.
10
[Interaction of mutations in the SUP45 (SUP1) gene in saccharomyces yeasts and their effect on protein structure].
Dokl Akad Nauk. 1993 Dec;333(5):658-60.

引用本文的文献

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Combining different mRNA capture methods to analyze the transcriptome: analysis of the Xenopus laevis transcriptome.结合不同的 mRNA 捕获方法来分析转录组：非洲爪蟾转录组分析。
PLoS One. 2013 Oct 15;8(10):e77700. doi: 10.1371/journal.pone.0077700. eCollection 2013.
2
Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division.拟南芥中真核生物释放因子1-1的共抑制影响细胞伸长和径向细胞分裂。
Plant Physiol. 2005 Sep;139(1):115-26. doi: 10.1104/pp.105.062695. Epub 2005 Aug 19.
3
Stop codon suppression via inhibition of eRF1 expression.

本文引用的文献

1
Ribosomal recessive suppressors cause a respiratory deficiency in yeast Saccharomyces cerevisiae.核糖体隐性抑制子会导致酿酒酵母出现呼吸缺陷。
Mol Gen Genet. 1982;185(2):319-23. doi: 10.1007/BF00330805.
2
Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.用于研究酵母中克隆基因表达的酵母启动子和乳糖操纵子Z融合体。
Methods Enzymol. 1983;101:181-91. doi: 10.1016/0076-6879(83)01013-7.
3
The use of Xenopus oocytes for the expression of cloned genes.非洲爪蟾卵母细胞在克隆基因表达中的应用。
通过抑制eRF1表达实现终止密码子抑制。
RNA. 2003 Jun;9(6):648-53. doi: 10.1261/rna.5280103.
4
Poly(A)-binding protein acts in translation termination via eukaryotic release factor 3 interaction and does not influence [PSI(+)] propagation.聚腺苷酸结合蛋白通过与真核释放因子3相互作用参与翻译终止，且不影响[PSI(+)]的传播。
Mol Cell Biol. 2002 May;22(10):3301-15. doi: 10.1128/MCB.22.10.3301-3315.2002.
5
Overexpression of the SUP45 gene encoding a Sup35p-binding protein inhibits the induction of the de novo appearance of the [PSI+] prion.编码一种与Sup35p结合蛋白的SUP45基因的过表达会抑制[PSI+]朊病毒从头出现的诱导。
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2400-5. doi: 10.1073/pnas.95.5.2400.
6
The catalytic subunit of protein phosphatase 2A associates with the translation termination factor eRF1.蛋白磷酸酶2A的催化亚基与翻译终止因子eRF1相关联。
EMBO J. 1996 Dec 16;15(24):7156-67.
7
Polypeptide chain termination in Saccharomyces cerevisiae.酿酒酵母中的多肽链终止
Curr Genet. 1994 May;25(5):385-95. doi: 10.1007/BF00351776.
8
Further analysis of cytoplasmic polyadenylation in Xenopus embryos and identification of embryonic cytoplasmic polyadenylation element-binding proteins.非洲爪蟾胚胎中细胞质多聚腺苷酸化的进一步分析及胚胎细胞质多聚腺苷酸化元件结合蛋白的鉴定。
Mol Cell Biol. 1994 Dec;14(12):7867-75. doi: 10.1128/mcb.14.12.7867-7875.1994.
9
The yeast translational allosuppressor, SAL6: a new member of the PP1-like phosphatase family with a long serine-rich N-terminal extension.酵母翻译别构抑制因子SAL6：PP1样磷酸酶家族的一个新成员，其N端富含丝氨酸且有长延伸序列。
Genetics. 1994 Nov;138(3):597-608. doi: 10.1093/genetics/138.3.597.
10
A human homologue to the yeast omnipotent suppressor 45 maps 100 kb centromeric to HLA-A.一种与酵母全能抑制因子45同源的人类基因定位于HLA - A着丝粒侧100 kb处。
Immunogenetics. 1995;41(6):380-2. doi: 10.1007/BF00163996.
Methods Enzymol. 1983;101:370-86. doi: 10.1016/0076-6879(83)01028-9.
4
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.非洲爪蟾早期胚胎发育中的一个主要转变：I. 囊胚中期细胞变化的特征与时间进程
Cell. 1982 Oct;30(3):675-86. doi: 10.1016/0092-8674(82)90272-0.
5
Suppressors in yeast.酵母中的抑制因子。
Curr Top Microbiol Immunol. 1974;64(0):1-47. doi: 10.1007/978-3-642-65848-8_1.
6
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.利用噬菌体T7 RNA聚合酶指导克隆基因的选择性高水平表达。
J Mol Biol. 1986 May 5;189(1):113-30. doi: 10.1016/0022-2836(86)90385-2.
7
Yeast/E. coli shuttle vectors with multiple unique restriction sites.具有多个独特限制性酶切位点的酵母/大肠杆菌穿梭载体。
Yeast. 1986 Sep;2(3):163-7. doi: 10.1002/yea.320020304.
8
Genetic control of translational fidelity in yeast: molecular cloning and analysis of the allosuppressor gene SAL3.
Mol Gen Genet. 1987 Dec;210(3):581-3. doi: 10.1007/BF00327216.
9
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.一种用于特异性基因的可控专一性表达的噬菌体T7 RNA聚合酶/启动子系统。
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8. doi: 10.1073/pnas.82.4.1074.
10
'Omnipotent' nonsense suppressors: new clues to an old puzzle.“全能”无义抑制基因：旧谜题的新线索
Trends Biochem Sci. 1988 Apr;13(4):120-3. doi: 10.1016/0968-0004(88)90062-x.