在非洲爪蟾中，一种受发育调控的信使核糖核酸的产物在结构和功能上与酿酒酵母中一种参与翻译保真度的蛋白质同源。
In Xenopus laevis, the product of a developmentally regulated mRNA is structurally and functionally homologous to a Saccharomyces cerevisiae protein involved in translation fidelity.
作者信息
Tassan J P, Le Guellec K, Kress M, Faure M, Camonis J, Jacquet M, Philippe M
机构信息
Département de Biologie et Génétique du Développement, CNRS URA 256, Université de Rennes I, France.
出版信息
Mol Cell Biol. 1993 May;13(5):2815-21. doi: 10.1128/mcb.13.5.2815-2821.1993.
We have performed a differential screen of a Xenopus egg cDNA library and selected two clones (Cl1 and Cl2) corresponding to mRNA which are specifically adenylated and recruited into polysomes after fertilization. Sequence analysis of Cl1 reveals that the corresponding protein is 67.5% identical (83% similar) to the product of the Saccharomyces cerevisiae SUP45 (also called SUP1 or SAL4) gene. This gene, when mutated, is an omnipotent suppressor of nonsense codons. When expressed in a sup45 mutant, the Xenopus Cl1 cDNA was able to suppress sup45-related phenotypes, showing that the structural homology reflects a functional homology. Our discovery of a structural and functional homolog in Xenopus cells implies that the function of SUP45 is not restricted to lower eukaryotes and that the SUP45 protein may perform a crucial cellular function in higher eukaryotes.
我们对非洲爪蟾卵cDNA文库进行了差异筛选，挑选出两个与受精后经特异性腺苷酸化并进入多核糖体的mRNA相对应的克隆（Cl1和Cl2）。对Cl1的序列分析显示，相应蛋白质与酿酒酵母SUP45（也称为SUP1或SAL4）基因的产物有67.5%的同一性（83%的相似性）。该基因发生突变时，是无义密码子的全能抑制子。当在sup45突变体中表达时，非洲爪蟾Cl1 cDNA能够抑制与sup45相关的表型，这表明结构同源性反映了功能同源性。我们在非洲爪蟾细胞中发现结构和功能同源物意味着SUP45的功能并不局限于低等真核生物，并且SUP45蛋白可能在高等真核生物中执行关键的细胞功能。
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