Crouzet M, Izgu F, Grant C M, Tuite M F
Biological Laboratory, University of Canterbury, Kent, UK.
Curr Genet. 1988 Dec;14(6):537-43. doi: 10.1007/BF00434078.
Allosuppressor (sal) mutations enhance the efficiency of the yeast ochre suppressor SUQ5 and define five unlinked loci, SAL1-SAL5. A number of sal4 mutants were isolated and found to have pleiotropic, allele;specific phenotypes, including hypersensitivity in vivo to paromomycin and other antibiotics that stimulate translational errors in yeast. To examine further the nature of the SAL4 gene product, the wild type SAL4 gene was isolated by complementation of a conditional lethal allele sal4-2, and demonstrated to be a single copy gene encoding a single 1.6 kb transcript. Restriction mapping and DNA hybridisation analysis were used to demonstrate that the SAL4 gene is identical to the previously identified omnipotent suppressor gene SUP45 (SUP1). Our results implicate the SAL4 gene product as playing a major role in maintaining translational accuracy in yeast.
同种抑制基因（sal）突变增强了酵母赭石型抑制基因SUQ5的效率，并确定了五个不连锁的基因座，即SAL1 - SAL5。分离出了许多sal4突变体，发现它们具有多效性、等位基因特异性表型，包括在体内对巴龙霉素和其他能刺激酵母翻译错误的抗生素过敏。为了进一步研究SAL4基因产物的性质，通过对条件致死等位基因sal4 - 2进行互补，分离出了野生型SAL4基因，并证明它是一个单拷贝基因，编码一个单一的1.6 kb转录本。通过限制性酶切图谱分析和DNA杂交分析表明，SAL4基因与先前鉴定的全能抑制基因SUP45（SUP1）相同。我们的结果表明，SAL4基因产物在维持酵母翻译准确性方面起主要作用。
