Duggan A W, Hope P J, Lang C W, Bjelke B
Department of Preclinical Veterinary Sciences, University of Edinburgh, UK.
Neurosci Lett. 1993 Jan 12;149(2):205-8. doi: 10.1016/0304-3940(93)90772-d.
As a test of the hypothesis that an animal responds to a severe peripheral painful stimulus by a central release of beta-endorphin, antibody microprobes were inserted stereotactically into the midbrain of urethane anesthetized rats. These microprobes bore antibodies to beta-endorphin immobilized to their outer surfaces. While microprobes were in the brain for periods of 10 to 30 min either no stimulus was delivered or alligator clamps were applied to both hind paws. Microprobes were then incubated with 125I-beta-endorphin. Quantitative image analysis of microprobe autoradiographs showed no differences between the no-stimulus and noxious-stimulus groups. Thus these experiments found no evidence for beta-endorphin release following a severe peripheral painful stimulus.
为了验证动物通过中枢释放β-内啡肽来应对严重外周疼痛刺激这一假说，将抗体微探针立体定向插入氨基甲酸乙酯麻醉大鼠的中脑。这些微探针的外表面固定有抗β-内啡肽的抗体。在微探针置于脑内10至30分钟的时间段内，要么不施加刺激，要么用鳄鱼夹夹两只后爪。然后将微探针与125I-β-内啡肽一起孵育。微探针放射自显影片的定量图像分析显示，无刺激组和有害刺激组之间没有差异。因此，这些实验没有发现严重外周疼痛刺激后β-内啡肽释放的证据。
