Voliva C F, Tsang S, Peterlin B M
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3408-12. doi: 10.1073/pnas.90.8.3408.
Defects in promoters of the nonexpressed DQA2, DQB2, and DOB genes from the class II major histo-compatibility complex were mapped by placing Z and X boxes of these silent genes into a synthetic DRA promoter. These conserved upstream sequences confer B-cell-specific and gamma-interferon-inducible expression to the DRA gene. Since DRA promoters containing the X box from the DQA2 gene and Z boxes from DQA2, DQB2, and DOB genes were neither expressed constitutively in B cells nor inducible by gamma interferon in fibroblastic cells, these conserved upstream sequences are implicated in the transcriptional defects of these silent genes.
通过将II类主要组织相容性复合体中未表达的DQA2、DQB2和DOB基因的启动子中的Z盒和X盒放入合成的DRA启动子中，对这些基因启动子的缺陷进行了定位。这些保守的上游序列赋予DRA基因B细胞特异性和γ干扰素诱导性表达。由于含有DQA2基因X盒以及DQA2、DQB2和DOB基因Z盒的DRA启动子既不在B细胞中组成性表达，也不在成纤维细胞中被γ干扰素诱导表达，因此这些保守的上游序列与这些沉默基因的转录缺陷有关。
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