HLA-DRα基因的B细胞特异性及γ干扰素诱导性调控
B-cell-specific and interferon-gamma-inducible regulation of the HLA-DR alpha gene.
作者信息
Tsang S Y, Nakanishi M, Peterlin B M
机构信息
Howard Hughes Medical Institute, University of California, San Francisco 94143.
出版信息
Proc Natl Acad Sci U S A. 1988 Nov;85(22):8598-602. doi: 10.1073/pnas.85.22.8598.
Abstract
We investigated the cis-acting sequences that function in the B-cell-specific and interferon-gamma-inducible expression of the HLA-DR alpha gene, a human class II major histocompatibility complex gene. The effects of 5' deletions on the activity of the DR alpha promoter and the influence of upstream DR alpha promoter elements on the activity of the herpes simplex virus thymidine kinase promoter were examined by a transient transfection assay in human B-, T-, and fibroblast cell lines. We show that the DR alpha gene is regulated by positive and negative cis-acting sequences between positions -1300 and +31 from the site of initiation of transcription. We also demonstrate that the DR alpha promoter sequences from positions -116 to -92 and from -136 to -80 are the minimal sequences required for conferring B-cell specificity and interferon-gamma inducibility upon the Herpes simplex virus thymidine kinase promoter, respectively.
摘要
我们研究了人类Ⅱ类主要组织相容性复合体基因HLA-DRα基因在B细胞特异性和γ干扰素诱导表达中起作用的顺式作用序列。通过在人B细胞、T细胞和成纤维细胞系中的瞬时转染试验，检测了5'端缺失对DRα启动子活性的影响以及上游DRα启动子元件对单纯疱疹病毒胸苷激酶启动子活性的影响。我们发现，DRα基因受转录起始位点上游-1300至+31位之间的正性和负性顺式作用序列调控。我们还证明，DRα启动子-116至-92位和-136至-80位的序列分别是赋予单纯疱疹病毒胸苷激酶启动子B细胞特异性和γ干扰素诱导性所需的最小序列。
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