Arata S, Newton C, Klein T W, Yamamoto Y, Friedman H
Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa 33612.
Proc Soc Exp Biol Med. 1993 May;203(1):26-9. doi: 10.3181/00379727-203-43568.
The ability of an opportunistic intracellular bacterial pathogen, Legionella pneumophila, to induce tumor necrosis factor (TNF) in macrophages from susceptible A/J or resistant BDF1 and BALB/c mice was determined. Cultures of peritoneal elicited macrophages from these mouse strains produced TNF in response to the Legionella. The TNF levels produced by the macrophages stimulated with either heat-killed Legionella vaccine or lipopolysaccharide were similar and dose dependent, although the amount of TNF produced by macrophages from permissive A/J mice was 2- to 4-fold higher than that produced by macrophages from the nonpermissive mice. Similar differences in TNF levels occurred when macrophages from either permissive or non-permissive mice were infected with viable Legionella. The TNF levels produced by the A/J mouse macrophages increased as a function of time after infection, with a peak of activity on Day 1 or 2, depending upon the initial concentration of the bacteria. Infection of the A/J mouse macrophages with avirulent Legionella resulted in induced levels comparable to those induced by a virulent strain. Although it is widely believed that TNF production by mouse macrophages is related to resistance to infections, the results of this study did not show a relationship between TNF production by macrophages in vitro and resistance versus susceptibility of the macrophage donor mouse strain to Legionella infection.
我们测定了机会性细胞内细菌病原体嗜肺军团菌在易感的A/J小鼠或抗性的BDF1和BALB/c小鼠的巨噬细胞中诱导肿瘤坏死因子（TNF）的能力。来自这些小鼠品系的腹腔诱导巨噬细胞培养物在接触嗜肺军团菌后产生了TNF。用热灭活的嗜肺军团菌疫苗或脂多糖刺激巨噬细胞产生的TNF水平相似且呈剂量依赖性，尽管来自易感性A/J小鼠的巨噬细胞产生的TNF量比来自非易感性小鼠的巨噬细胞高2至4倍。当来自易感性或非易感性小鼠的巨噬细胞感染活的嗜肺军团菌时，TNF水平也出现了类似的差异。A/J小鼠巨噬细胞产生的TNF水平随着感染后的时间而增加，根据细菌的初始浓度，在第1天或第2天达到活性峰值。用无毒力的嗜肺军团菌感染A/J小鼠巨噬细胞所诱导的水平与用有毒力菌株诱导的水平相当。尽管人们普遍认为小鼠巨噬细胞产生TNF与抗感染能力有关，但本研究结果并未显示体外巨噬细胞产生TNF与巨噬细胞供体小鼠品系对嗜肺军团菌感染的抗性或易感性之间存在关联。
