Littig J S, Nieman T A
Department of Chemistry, University of Illinois, Urbana 61801.
J Biolumin Chemilumin. 1993 Jan-Feb;8(1):25-31. doi: 10.1002/bio.1170080106.
Decomposition of phenyl acridinium-9-carboxylate is monitored using electrogenerated chemiluminescence in a flow system. The formation of the pseudobase from the acridinium ester [AE] is described by rate = k'1[AE] + k''1[AE][OH-]0.5, where k'1 = 0.020 +/- 0.006 s-1 and k''1 = 2.1 +/- 0.8 (L/mol)-0.5 s-1. Irreversible decomposition of the pseudobase is described by rate = k'2[AE][OH-], where k'2 = 20.1 +/- 3.8 (L/mol s). These kinetic equations, plus measurement of variation in emission intensity for constant acridinium ester concentration, are used to predict the resulting emission intensity v. pH behaviour given various contact times (in the 0.25 to 25 s range) for the acridinium ester to be in an alkaline solution prior to initiation of the chemiluminescence reaction.
在流动体系中，利用电化学发光监测9-羧酸苯基吖啶鎓的分解。吖啶酯[AE]形成假碱的速率由公式rate = k'1[AE] + k''1[AE][OH-]0.5描述，其中k'1 = 0.020 ± 0.006 s-1，k''1 = 2.1 ± 0.8 (L/mol)-0.5 s-1。假碱的不可逆分解由公式rate = k'2[AE][OH-]描述，其中k'2 = 20.1 ± 3.8 (L/mol s)。这些动力学方程，加上在吖啶酯浓度恒定的情况下对发射强度变化的测量，用于预测在化学发光反应开始前，吖啶酯在碱性溶液中处于不同接触时间（0.25至25秒范围内）时的发射强度与pH值的关系。
