Feldman S R, Trojanowska M, Smith E A, Leroy E C
Department of Dermatology, Medical University of South Carolina, Charleston.
Am J Med Sci. 1993 Apr;305(4):203-7. doi: 10.1097/00000441-199304000-00002.
Differences in the responses to growth factors of normal fibroblasts and scleroderma fibroblasts have been demonstrated previously. Because human dermal fibroblasts are heterogeneous populations, whether known differences between papillary and reticular dermal fibroblasts could account for the noted differences between normal and scleroderma fibroblasts was investigated. Papillary dermal fibroblasts were grown from a dermatome section of normal skin from an adult donor. Reticular dermal fibroblasts were cultured from punch biopsy specimens taken from the same location. In vitro, papillary dermal fibroblasts proliferated more rapidly, had a higher mitotic index and reached greater density at confluence, confirming previous observations. The reticular dermal fibroblasts were more dendritic. Reticular dermal fibroblasts had higher rates of tritiated thymidine uptake and larger increases in mitotic index in response to isoforms of platelet-derived growth factor (PDGF). The characteristic response of scleroderma fibroblasts, potentiation of the mitogenicity of PDGF AA by transforming growth factor-beta (TGF-beta), was not observed in either cell type. Therefore, the phenotypic characteristics of scleroderma fibroblasts cannot be explained by an unusual admixture of papillary and reticular fibroblasts.
正常成纤维细胞和硬皮病成纤维细胞对生长因子的反应差异此前已有报道。由于人皮肤成纤维细胞是异质群体，因此研究了乳头层和网状层皮肤成纤维细胞之间已知的差异是否可以解释正常成纤维细胞和硬皮病成纤维细胞之间的显著差异。乳头层皮肤成纤维细胞取自成年供体正常皮肤的皮片。网状层皮肤成纤维细胞从同一部位的打孔活检标本中培养。在体外，乳头层皮肤成纤维细胞增殖更快，有丝分裂指数更高，汇合时达到更高的密度，这证实了先前的观察结果。网状层皮肤成纤维细胞的树突状结构更明显。网状层皮肤成纤维细胞对血小板衍生生长因子（PDGF）异构体的氚化胸腺嘧啶摄取率更高，有丝分裂指数增加更大。在这两种细胞类型中均未观察到硬皮病成纤维细胞的特征性反应，即转化生长因子-β（TGF-β）增强PDGF AA的促有丝分裂活性。因此，硬皮病成纤维细胞的表型特征不能用乳头层和网状层成纤维细胞的异常混合来解释。
