Tada A, Sasaki H, Nakamura J, Yoshihama M, Terashima Y
Department of Obstetrics and Gynecology, Jikei University School of Medicine, Tokyo, Japan.
J Steroid Biochem Mol Biol. 1993 Mar;44(4-6):661-6. doi: 10.1016/0960-0760(93)90276-3.
Human endometrial and breast carcinoma cell lines were examined for aromatase activity and the effects of sex steroids (estradiol and testosterone) on DNA synthesis. Aromatase activity was high (greater than 500 fmol/10(7) cells/24 h) in the cell lines MCF-7 and OMC-2, moderate (100-499 fmol/10(7) cells/24 h) in the cell lines HEC-59 and Ishikawa, and low (less than 100 fmol/10(7) cells/24 h) in the HHUA cell line. A substantial stimulation of DNA synthesis by estradiol (10(-9)M) was observed in cell lines HEC-59, OMC-2, and MCF-7, with an increase in [3H]thymidine uptake of over 250%. The Ishikawa cell line was stimulated moderately (115-249%). No estradiol-induced increase in DNA synthesis was observed in HHUA. Responsiveness of DNA synthesis to testosterone was observed in cell lines that showed the greatest response to estradiol, namely HEC-59, OMC-2, and MCF-7. Otherwise, estrogen-responsiveness did not always correlated with a significant aromatase activity. These data suggest that some but not all endometrial carcinomas may possess an aromatase-dependent growth stimulating system.
对人子宫内膜癌细胞系和乳腺癌细胞系进行了芳香化酶活性以及性类固醇（雌二醇和睾酮）对DNA合成影响的检测。在MCF - 7和OMC - 2细胞系中，芳香化酶活性较高（大于500 fmol/10⁷细胞/24小时）；在HEC - 59和Ishikawa细胞系中，活性中等（100 - 499 fmol/10⁷细胞/24小时）；而在HHUA细胞系中，活性较低（小于100 fmol/10⁷细胞/24小时）。在HEC - 59、OMC - 2和MCF - 7细胞系中，观察到雌二醇（10⁻⁹M）对DNA合成有显著刺激作用，[³H]胸腺嘧啶核苷摄取量增加超过250%。Ishikawa细胞系受到中度刺激（115 - 249%）。在HHUA细胞系中未观察到雌二醇诱导的DNA合成增加。在对雌二醇反应最强烈的细胞系（即HEC - 59、OMC - 2和MCF - 7）中，观察到了DNA合成对睾酮的反应。此外，雌激素反应性并不总是与显著的芳香化酶活性相关。这些数据表明，部分而非全部子宫内膜癌可能具有一种依赖芳香化酶的生长刺激系统。
