Yamamoto T, Kitawaki J, Urabe M, Honjo H, Tamura T, Noguchi T, Okada H, Sasaki H, Tada A, Terashima Y
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan.
J Steroid Biochem Mol Biol. 1993 Mar;44(4-6):463-8. doi: 10.1016/0960-0760(93)90251-q.
Aromatase, estrone (E1) sulfatase and E1 sulfotransferase activities were examined in endometrium and endometrial cancer tissue preparations. Aromatase and E1 sulfatase activities in endometrial cancer tissues were found to be significantly higher than in normal endometrial tissues. However, E1 sulfotransferase activity did not differ between benign and malignant tissue. We also examined the effect of testosterone (T) on aromatase activity and tritiated thymidine uptake (DNA synthesis) in various cultured cervical or corpus endometrial cancer cell lines (OMC-4, HHUA, Ishikawa, HEC-59). The results demonstrated that only the HEC-59 cell line had high aromatase activity and increased its DNA synthesis in response to T. This increase of DNA synthesis by T was not suppressed by simultaneous addition of cyproterone acetate, but was by tamoxifen. These data suggest that in situ estrogen production in endometrial cancer tissue is biologically important and that aromatase in cancer cells may contribute partially to cell proliferation if androgen substrate is provided.
在子宫内膜及子宫内膜癌组织样本中检测了芳香化酶、雌酮（E1）硫酸酯酶及E1磺基转移酶的活性。结果发现，子宫内膜癌组织中的芳香化酶和E1硫酸酯酶活性显著高于正常子宫内膜组织。然而，良性和恶性组织中的E1磺基转移酶活性并无差异。我们还研究了睾酮（T）对各种培养的宫颈或子宫内膜癌细胞系（OMC-4、HHUA、石川、HEC-59）中芳香化酶活性及氚标记胸腺嘧啶核苷摄取（DNA合成）的影响。结果表明，只有HEC-59细胞系具有较高的芳香化酶活性，并且其DNA合成会因T而增加。T引起的DNA合成增加不会被同时添加的醋酸环丙孕酮抑制，但会被他莫昔芬抑制。这些数据表明，子宫内膜癌组织中的原位雌激素生成具有生物学重要性，并且如果有雄激素底物，癌细胞中的芳香化酶可能部分促成细胞增殖。
