Pillai R, Kannan S, Chandran G J
Regional Cancer Centre, Thiruvananthapuram, Kerala, India.
Natl Med J India. 1993 Mar-Apr;6(2):71-5.
Immunohistochemistry has become an important tool for tumour typing and has the potential to improve the histopathological diagnosis of neoplasms. However, the large number of publications on the different aspects of the technology are often contradictory. In many instances the immunochemical reactions may not be reproducible and can create problems in the diagnosis of certain tumours. The use of optimal materials and techniques enhance the sensitivity, specificity and reproducibility of these methods. In this article we discuss the common problems of tissue preparation, and antigenic variation, distribution and presentation. However, immunopathological diagnosis has to be accompanied by an in-depth and fundamental understanding of basic immunochemistry and diagnostic immunohistochemistry has to be interpreted in the context of a standard clinical and histopathological examination.
免疫组织化学已成为肿瘤分型的重要工具，并且有可能改善肿瘤的组织病理学诊断。然而，关于该技术不同方面的大量出版物往往相互矛盾。在许多情况下，免疫化学反应可能无法重复，并且会在某些肿瘤的诊断中产生问题。使用最佳材料和技术可提高这些方法的敏感性、特异性和可重复性。在本文中，我们讨论了组织制备、抗原变异、分布和呈递等常见问题。然而，免疫病理学诊断必须伴随着对基础免疫化学的深入和根本理解，并且诊断性免疫组织化学必须在标准临床和组织病理学检查的背景下进行解释。
