Watt D J, Karasinski J, England M A
Department of Anatomy, Charing Cross and Westminster Medical School, London, UK.
J Muscle Res Cell Motil. 1993 Feb;14(1):121-32. doi: 10.1007/BF00132186.
C2 mouse myogenic cells carrying the lacZ gene coding for beta-galactosidase (beta-gal) were injected into the tibialis anterior muscle of dystrophin-deficient mdx mice. Introduced cells were shown to have been incorporated into fibres of the injected muscle by virtue of the colocalization of beta-gal and dystrophin within them. Synthetic Nuclepore membrane inserted between the injected tibialis anterior and adjacent extensor digitorum longus muscle permitted the visualization of cells migrating between the two muscles through the pores of the membrane. Although the exact nature of the cells passing through the Nuclepore could not be determined by this method, they were thought to include implanted myogenic cells. Evidence for this was gained by the presence of beta-gal/dystrophin positive fibres within the extensor digitorum longus. Incorporation of cells into the adjacent extensor digitorum longus was greater in animals where this muscle had been autografted by the cutting and resuturing of the distal tendon. Autografted extensor digitorum longi differed from those which had not been subject to this procedure, by undergoing extensive fibre degeneration followed by regeneration, and further by the stripping of their surrounding epimysial covering. Implanted cells substantially participated in extensor digitorum longus fibre formation in these mice, up to 31% of their fibres 3 weeks after implantation coexpressing both the introduced lacZ gene product and the dystrophin gene product, the latter not normally expressed within the fibres of this myopathic recipient.
将携带编码β-半乳糖苷酶（β-gal）的lacZ基因的C2小鼠成肌细胞注射到缺乏抗肌萎缩蛋白的mdx小鼠的胫前肌中。通过β-gal和抗肌萎缩蛋白在细胞内的共定位，证明引入的细胞已整合到注射肌肉的纤维中。插入在注射的胫前肌和相邻的趾长伸肌之间的合成核孔膜，使得能够观察到细胞通过膜孔在两块肌肉之间迁移。尽管通过这种方法无法确定穿过核孔的细胞的确切性质，但认为它们包括植入的成肌细胞。在趾长伸肌中存在β-gal/抗肌萎缩蛋白阳性纤维，从而获得了这方面的证据。在通过切断和重新缝合远端肌腱对该肌肉进行自体移植的动物中，细胞整合到相邻趾长伸肌中的情况更为明显。自体移植的趾长伸肌与未进行此操作的肌肉不同，它经历了广泛的纤维变性，随后再生，并且其周围的肌外膜覆盖物被剥离。在这些小鼠中，植入的细胞大量参与了趾长伸肌纤维的形成，植入后3周，其纤维中高达31%同时共表达引入的lacZ基因产物和抗肌萎缩蛋白基因产物，后者在这种肌病受体的纤维中通常不表达。
