Hardy R, Reynolds R
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, UK.
J Neurosci Res. 1993 Apr 1;34(5):589-600. doi: 10.1002/jnr.490340510.
The development of cells of the oligodendroglial lineage, from immature progenitor to myelinating cells, occurs largely in a neuronal environment, yet little is known about specific interactions between these 2 cell types. We have tested the effects of medium conditioned by cultures of rat cerebral cortical neurons (CCM), cerebellar granule interneurons (GCM), and a dorsal root ganglion derived cell line (NDCM) on cells of the oligodendroglial lineage in culture. Different stages of the lineage were defined using the cell surface antigens GD3, 04, and GalC. CCM and NDCM were mitogenic for the early GD3+/04- oligodendroglial progenitor, whereas GCM was mitogenic for the later GD3+/04+ stage. Neutralising antibodies to PDGF and bFGF were able to eliminate the mitogenic activity of NDCM and GCM, respectively, but did not abolish the mitogenic effect of CCM. We have demonstrated that neurons in primary culture from distinct CNS regions exert different influences on cells of the oligodendroglial lineage, and specifically that cortical neurons release an unknown mitogen for GD3+/04- oligodendroglial progenitors.
少突胶质细胞系的细胞从未成熟祖细胞发育为髓鞘形成细胞，这一过程主要发生在神经元环境中，但对于这两种细胞类型之间的具体相互作用却知之甚少。我们测试了大鼠大脑皮质神经元培养物（CCM）、小脑颗粒中间神经元培养物（GCM）和背根神经节衍生细胞系（NDCM）所条件化的培养基对培养中的少突胶质细胞系细胞的影响。利用细胞表面抗原GD3、O4和半乳糖脑苷脂（GalC）定义了该细胞系的不同阶段。CCM和NDCM对早期GD3+/O4-少突胶质祖细胞有促有丝分裂作用，而GCM对后期GD3+/O4+阶段有促有丝分裂作用。针对血小板衍生生长因子（PDGF）和碱性成纤维细胞生长因子（bFGF）的中和抗体分别能够消除NDCM和GCM的促有丝分裂活性，但并未消除CCM的促有丝分裂作用。我们已经证明，来自不同中枢神经系统区域的原代培养神经元对少突胶质细胞系细胞有不同影响，具体而言，皮质神经元会释放一种未知的有丝分裂原，作用于GD3+/O4-少突胶质祖细胞。
