Pál M, Tóth A, Wu C H, Tucker R A, Johnson P C
Department of Physiology, University of Arizona, Tucson 85724.
Microvasc Res. 1993 Jan;45(1):95-105. doi: 10.1006/mvre.1993.1009.
A system has been developed for intravital microscopy studies that records optical signals at different tissue sites together with X-Y coordinates within an area of up to 100 cm2. Z axis coordinates can also be recorded. The system utilizes a computer and a motor-driven microscope stage to rapidly and repetitively move between selected sites. The system can also be used to map topological features and geometry. The application of the system to study spatial heterogeneity of changes in tissue metabolism with time and to map vascular network architecture is described.
已经开发出一种用于活体显微镜研究的系统，该系统可记录不同组织部位的光信号以及高达100平方厘米区域内的X-Y坐标。Z轴坐标也可以记录。该系统利用计算机和电动显微镜载物台在选定部位之间快速重复移动。该系统还可用于绘制拓扑特征和几何图形。本文描述了该系统在研究组织代谢随时间变化的空间异质性以及绘制血管网络结构方面的应用。
