Toth A, Tischler M E, Pal M, Koller A, Johnson P C
Department of Physiology, University of Arizona, Tucson 85724.
J Appl Physiol (1985). 1992 Jul;73(1):296-306. doi: 10.1152/jappl.1992.73.1.296.
An in vivo microscope system has been developed that can measure fluorescence emission and/or light absorption at up to five wavelengths in a tissue area of 18-30 microns diam while imaging adjacent microcirculatory vessels with a video system. The system also incorporates a computer-controlled stage and data acquisition system for rapid and repeated measurements from a number of tissue sites. The tissue area monitored for fluorescence or absorption can be defined further by a confocal arrangement of the microscope optics. Tests of the system for NADH fluorescence measurements show good agreement between the fluorescence at 450 nm and NADH concentration in vitro and in skeletal muscle. The instrument can also be used simultaneously for spectrophotometric determination of O2 saturation and hematocrit in microcirculatory vessels. In vitro tests indicate suitable accuracy for such measurements. The open architecture and modular arrangement of the instrument facilitates its use for a variety of simultaneous measurements of parenchymal cell and microcirculatory function.
已开发出一种体内显微镜系统，该系统能够在直径为18 - 30微米的组织区域内测量多达五个波长的荧光发射和/或光吸收，同时通过视频系统对相邻的微循环血管进行成像。该系统还配备了计算机控制的载物台和数据采集系统，以便对多个组织部位进行快速重复测量。用于监测荧光或吸收的组织区域可通过显微镜光学系统的共聚焦配置进一步界定。该系统用于NADH荧光测量的测试表明，450纳米处的荧光与体外及骨骼肌中的NADH浓度之间具有良好的一致性。该仪器还可同时用于分光光度法测定微循环血管中的氧饱和度和血细胞比容。体外测试表明此类测量具有合适的精度。该仪器的开放式架构和模块化配置便于其用于对实质细胞和微循环功能进行多种同步测量。
