Röder M S, Lapitan N L, Sorrells M E, Tanksley S D
Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY.
Mol Gen Genet. 1993 Apr;238(1-2):294-303. doi: 10.1007/BF00279558.
Barley (Hordeum vulgare L.) telomeres were investigated by means of pulsed field gel electrophoresis (PFGE) and in situ hybridization. In situ hybridization showed that a tandemly repeated satellite sequence has a subtelomeric location, and is present at thirteen of the fourteen chromosome ends. PFGE revealed that this satellite sequence is physically close to the telomeric repeat. Pulsed field gel electrophoresis was then used for segregation analysis and linkage mapping of several telomeric and satellite loci in a segregating doubled-haploid population. The telomeric repeat displayed a hypervariable segregation pattern with new alleles occurring in the progeny. Eight satellite and telomeric sites were mapped on an restriction fragment length polymorphism (RFLP)-map of barley, defining the ends of chromosome arms 1L, 2S, 3L, 4S, 4L, 5S and 6. One satellite locus mapped to an interstitial site on the long arm of chromosome 3. The physical location of this locus was confirmed by in situ hybridization to wheat/barley addition line 3.
利用脉冲场凝胶电泳（PFGE）和原位杂交技术对大麦（Hordeum vulgare L.）的端粒进行了研究。原位杂交显示，一个串联重复的卫星序列位于亚端粒位置，并且存在于14个染色体末端中的13个末端。PFGE结果表明，该卫星序列在物理位置上靠近端粒重复序列。随后，脉冲场凝胶电泳被用于一个分离加倍单倍体群体中几个端粒和卫星位点的分离分析及连锁作图。端粒重复序列呈现出高变分离模式，后代中出现了新的等位基因。8个卫星和端粒位点被定位在大麦的限制性片段长度多态性（RFLP）图谱上，确定了染色体臂1L、2S、3L、4S、4L、5S和6的末端。一个卫星位点定位到了3号染色体长臂的一个中间位置。通过与小麦/大麦附加系3进行原位杂交，证实了该位点的物理位置。
