Röder M S, Sorrells M E, Tanksley S D
Institute for Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Genome. 1995 Feb;38(1):153-7. doi: 10.1139/g95-019.
Pulsed-field gel electrophoresis was used to study the variability of clustered tandemly repeated sequences in barley. Twelve spring barley cultivars were investigated with a heterologous 5S DNA probe and the 118 base pair barley satellite DNA probe HVT01. On a per fragment basis, the 5S probe was 5 times and the barley satellite probe 6.7 times more variable than single- or low-copy RFLP markers, demonstrating their usefulness for cultivar distinction.
采用脉冲场凝胶电泳研究大麦中串联重复序列的变异性。用异源5S DNA探针和118碱基对的大麦卫星DNA探针HVT01对12个春大麦品种进行了研究。以每个片段为基础，5S探针的变异性比单拷贝或低拷贝RFLP标记高5倍，大麦卫星探针高6.7倍，表明它们在区分品种方面的有效性。
