Jensen A L, Petersen M B, Houe H
Department of Clinical Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Zentralbl Veterinarmed A. 1993 Mar;40(2):111-7. doi: 10.1111/j.1439-0442.1993.tb00607.x.
The study reports on a spectrophotometric assay for the determination of serum fructosamine concentration. The assay was evaluated for use in bovine serum samples by assessment of the precision, accuracy and detectability. A reference interval for the bovine serum fructosamine concentration was also established. Further, the effect of acute changes in blood glucose on serum fructosamine concentration as well as the diurnal variations of the serum fructosamine concentration were investigated. The main conclusions can be summarized as follows: Determination of bovine serum fructosamines may be achieved by a precise and accurate assay with a detection limit well below the serum fructosamine concentration normally found in bovine sera. As a reference interval for the bovine serum fructosamine concentration 213.4 mumol/l to 265.0 mumol/l may be used. Serum fructosamine concentration is not affected by acute changes in blood glucose, and it displays no significant diurnal variation in cows.
该研究报告了一种用于测定血清果糖胺浓度的分光光度法。通过评估精密度、准确度和可检测性，对该检测方法在牛血清样本中的应用进行了评估。还建立了牛血清果糖胺浓度的参考区间。此外，研究了血糖急性变化对血清果糖胺浓度的影响以及血清果糖胺浓度的昼夜变化。主要结论可总结如下：通过一种精密度高、准确度好且检测限远低于牛血清中通常发现的血清果糖胺浓度的检测方法，可以实现牛血清果糖胺的测定。牛血清果糖胺浓度的参考区间可采用213.4 μmol/l至265.0 μmol/l。血清果糖胺浓度不受血糖急性变化的影响，并且在奶牛中未显示出明显的昼夜变化。
