Judmaier G, Meyersbach P, Weiss G, Wachter H, Reibnegger G
Department of Internal Medicine, University Hospital, Innsbruck, Austria.
Am J Gastroenterol. 1993 May;88(5):706-11.
Neopterin, a product of human monocytes/macrophages stimulated by gamma-interferon, was previously found to be correlated with clinical activity of Crohn's disease. The present investigation attempted to identify more precisely the role of neopterin for assessment of clinical activity in a multivariate setting. A broad panel of clinical and laboratory variables was obtained on 76 patients. Neopterin was determined in urine by high performance liquid chromatography. Using a 4-point clinical activity score as dependent variable, multivariate linear discriminant analyses were performed in order to identify subsets of statistically independent laboratory activity indices. Neopterin was shown to be an independent indicator for clinical activity. The significant variables were then tested together with the eight constituent variables of the Crohn's Disease Activity Index. The resulting model, which consisted of the three laboratory variables--erythrocyte sedimentation rate, serum protein and neopterin, together with pain score--was further subjected to two multivariate techniques for comparison: polychotomous logistic regression analysis and the technique of classification and regression trees. By all methods, neopterin was included into the multivariate models, confirming that its determination aids in the assessment of clinical activity in Crohn's disease.
新蝶呤是γ-干扰素刺激人单核细胞/巨噬细胞产生的一种产物，此前发现它与克罗恩病的临床活动相关。本研究试图在多变量环境中更精确地确定新蝶呤在评估临床活动中的作用。对76例患者获取了一系列广泛的临床和实验室变量。采用高效液相色谱法测定尿液中的新蝶呤。以4分制临床活动评分作为因变量，进行多变量线性判别分析，以确定具有统计学独立性的实验室活动指标子集。结果表明，新蝶呤是临床活动的一个独立指标。然后将这些显著变量与克罗恩病活动指数的八个构成变量一起进行测试。最终得到的模型由三个实验室变量——红细胞沉降率、血清蛋白和新蝶呤，以及疼痛评分组成，该模型进一步采用两种多变量技术进行比较：多分类逻辑回归分析和分类与回归树技术。通过所有方法，新蝶呤都被纳入多变量模型，证实了其测定有助于评估克罗恩病的临床活动。
