Faber K A, Ayyash L, Dixon S, Hughes C L
Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina 27710.
Biol Reprod. 1993 May;48(5):947-51. doi: 10.1095/biolreprod48.5.947.
The effects of neonatal diethylstilbestrol (DES) exposure on the volume of the sexually dimorphic nucleus of the preoptic area of the hypothalamus (SDN-POA) and on GnRH-stimulated LH secretion were investigated in castrated female rats of known anogenital distance (AGD) at birth. The AGD was measured in females on the day of birth, and 0.1 microgram DES or corn oil was injected from Days 1 through 10 of life. The volume of the SDN-POA was significantly larger in animals that had received DES than in those that had received corn oil. The largest SDN-POA volumes were seen in DES-treated animals that had long (> 1.4 mm) AGDs, and smallest volumes in corn oil-treated females that had short (< or = to 1.4 mm) AGDs. Within treatment groups, animals with longer AGDs had significantly larger SDN-POA volumes than those with short AGDs. Within AGD subgroups, the effect of DES was similar in that the percentage increase in SDN-POA volume was equivalent. Pituitary responsiveness to GnRH was greater in corn oil-treated females with long AGD than in similarly treated females with short AGD. DES treatment blunted LH secretion in both AGD subgroups, but the increased LH secretion was preserved in rats with long AGD. The results indicate that the individual effects of the postnatal environment depend on the androgenicity of the intrauterine microenvironment. Further, variations in the individual's response to potential environmental hazards may be predicted by antecedent intrauterine events.
研究了新生大鼠暴露于己烯雌酚（DES）对下丘脑视前区性二态核（SDN-POA）体积以及对促性腺激素释放激素（GnRH）刺激的促黄体生成素（LH）分泌的影响，实验对象为出生时已知肛门生殖距（AGD）的去势雌性大鼠。在雌性大鼠出生当天测量其AGD，并在出生后第1天至第10天注射0.1微克DES或玉米油。接受DES的动物的SDN-POA体积显著大于接受玉米油的动物。在接受DES治疗且AGD长（>1.4毫米）的动物中观察到最大的SDN-POA体积，而在接受玉米油治疗且AGD短（≤1.4毫米）的雌性动物中观察到最小的体积。在各治疗组中，AGD长的动物的SDN-POA体积显著大于AGD短的动物。在AGD亚组中，DES的作用相似，即SDN-POA体积的增加百分比相当。AGD长的接受玉米油治疗的雌性大鼠的垂体对GnRH的反应性高于AGD短的类似治疗的雌性大鼠。DES治疗使两个AGD亚组的LH分泌均减弱，但AGD长的大鼠中LH分泌的增加得以保留。结果表明，出生后环境的个体效应取决于子宫内微环境的雄激素性。此外，个体对潜在环境危害的反应差异可能可由先前的子宫内事件预测。
