Uguccioni M, Meliconi R, Nesci S, Lucarelli G, Ceska M, Gasbarrini G, Facchini A
Laboratorio Immunologia e Genetica, Istituto di Ricerca Codivilla-Putti, IOR, Bologna, Italy.
Blood. 1993 May 1;81(9):2252-6.
Neutrophil chemotactic and functional defects occur in beta-thalassemia and in patients after bone marrow transplantation (BMT). Interleukin-8 (IL-8) is a novel chemotactic and activating peptide for neutrophils and can be detected in the circulation. IL-8 serum concentrations were evaluated in 30 beta-thalassemic patients before and after BMT. Serial samples from 16 patients were also studied. Fourteen sera from healthy children, 43 patients with chronic viral hepatitis, 16 patients on chronic transfusion treatment for various hematologic disorders, and 28 healthy adults were studied as controls. IL-8 was evaluated by an enzyme-linked immunosorbent assay. Patients with beta-thalassemia had higher IL-8 concentrations than did normal controls, patients with liver disease, and patients on chronic transfusion. beta-Thalassemic patients with severe liver siderosis and fibrosis had the highest IL-8 concentrations. After BMT in patients with successful engraftment, IL-8 concentrations decreased significantly. In contrast, in patients with acute graft-versus-host disease (GVHD), IL-8 concentrations were not statistically different from the concentrations found before BMT and were higher than in patients with no complications and patients with graft rejection. IL-8 may play a part in the immune dysregulation that occurs in beta-thalassemia and may be involved in the immune mechanisms leading to GVHD.
β地中海贫血患者以及骨髓移植（BMT）后的患者会出现中性粒细胞趋化和功能缺陷。白细胞介素-8（IL-8）是一种新型的中性粒细胞趋化和激活肽，可在循环中检测到。对30例β地中海贫血患者在BMT前后的IL-8血清浓度进行了评估。还对16例患者的系列样本进行了研究。研究了14名健康儿童的血清、43例慢性病毒性肝炎患者、16例因各种血液系统疾病接受慢性输血治疗的患者以及28名健康成年人作为对照。通过酶联免疫吸附测定法评估IL-8。β地中海贫血患者的IL-8浓度高于正常对照、肝病患者和慢性输血患者。患有严重肝脏铁沉积和纤维化的β地中海贫血患者的IL-8浓度最高。在移植成功的患者进行BMT后，IL-8浓度显著下降。相比之下，在患有急性移植物抗宿主病（GVHD）的患者中，IL-8浓度与BMT前的浓度无统计学差异，且高于无并发症患者和移植排斥患者。IL-8可能参与了β地中海贫血中发生的免疫失调，并且可能与导致GVHD的免疫机制有关。
