Mpofu S M, Masendu H T, Kanyimo K H, Mtetwa C
Malaria Section, Blair and de Beers Research Laboratories, Causeway, Zimbabwe.
Med Vet Entomol. 1993 Apr;7(2):122-6. doi: 10.1111/j.1365-2915.1993.tb00664.x.
A laboratory colony of the mosquito Anopheles quadriannulatus was established from a wild population occurring sympatrically with An.arabiensis in Zimbabwe. These sibling species are members of the An.gambiae Giles complex and were distinguished primarily by means of their specific polytene chromosome banding patterns. By using an ox-baited trap, we sampled selectively for the more zoophilic An.quadriannulatus. It was confirmed that An.quadriannulatus has the diagnostic slow allozyme of aspartate aminotransferase (AAT95/95). In a mixed population under laboratory conditions, An.arabiensis displaced An.quadriannulatus within eight generations, without introgression. Colonization of An.quadriannulatus was facilitated by pooling the progeny from wild-caught mothers of confirmed identity and by using a specially adapted cage to promote mating.
从津巴布韦与阿拉伯按蚊同域分布的野生种群中建立了四斑按蚊的实验室种群。这些姊妹种是冈比亚按蚊吉尔斯复合体的成员，主要通过其特定的多线染色体带型来区分。通过使用诱捕牛的诱捕器，我们有选择地采集了更嗜动物血的四斑按蚊。已证实四斑按蚊具有诊断性的天冬氨酸转氨酶慢等位酶（AAT95/95）。在实验室条件下的混合种群中，阿拉伯按蚊在八代内取代了四斑按蚊，且没有基因渗入。通过汇集已确认身份的野生捕获雌蚊的后代，并使用经过特殊改造的笼子来促进交配，有助于四斑按蚊的定殖。
