Hunt R H, Coetzee M, Fettene M
Department of Tropical Diseases, School of Pathology of the South African Institute for Medical Research, Johannesburg, South Africa.
Trans R Soc Trop Med Hyg. 1998 Mar-Apr;92(2):231-5. doi: 10.1016/s0035-9203(98)90761-1.
Historically, members of the Anopheles gambiae complex from Ethiopia have been identified chromosomally as either A. arabiensis or A. quadriannulatus. Recent collections from the Jimma area in Ethiopia, southwest of Addis Ababa, revealed 29 specimens of A. quadriannulatus based on the standard polymerase chain reaction (PCR) identification method. 'Wild' females were induced to lay eggs and the progeny reared as individual families. Resulting adults were cross-mated to a laboratory colony strain of A. quadriannulatus originating from the Kruger National Park, South Africa. Hybrid progeny were obtained only from the colony female x Ethiopian male cross. This cross produced a female/male sex ratio of 0.48. Male offspring were sterile and ovarian polytene chromosomes from hybrid females showed typical asynapsis as expected in interspecific crosses within the A. gambiae complex. The X chromosomes, although apparently having homosequential banding patterns, were usually totally asynapsed. All autosomes were homosequential. The lack of inversion heterozygotes, in both the wild and hybrid samples, may simply be a reflection of the small sample size. Until such time as the Ethiopian species can be formally described and assigned a scientific name, it is provisionally designated Anopheles quadriannulatus species B because of its close similarity to this species.
从历史上看，埃塞俄比亚的冈比亚按蚊复合体成员通过染色体鉴定为阿拉伯按蚊或四斑按蚊。最近在埃塞俄比亚亚的斯亚贝巴西南部的吉马地区采集的样本，基于标准聚合酶链反应（PCR）鉴定方法，发现了29只四斑按蚊标本。“野生”雌蚊被诱导产卵，后代作为单个家庭饲养。所得成虫与源自南非克鲁格国家公园的四斑按蚊实验室群体品系进行杂交交配。仅从群体雌蚊×埃塞俄比亚雄蚊的杂交中获得了杂交后代。这种杂交产生的雌雄性别比为0.48。雄性后代不育，杂交雌蚊的卵巢多线染色体显示出典型的异联会，这在冈比亚按蚊复合体的种间杂交中是预期的。X染色体虽然显然具有同源序列带型，但通常完全异联会。所有常染色体都是同源序列的。在野生和杂交样本中缺乏倒位杂合子，可能仅仅是样本量小的反映。在埃塞俄比亚的这个物种能够被正式描述并赋予一个科学名称之前，由于它与四斑按蚊非常相似，暂时将其指定为四斑按蚊B种。
