Garrett L R, Ackland-Berglund C E, Jones C J, Cuchens M A
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505.
Exp Cell Res. 1993 Apr;205(2):416-21. doi: 10.1006/excr.1993.1107.
The effects of 12-O-tetradecanoylphorbol-13-acetate (TPA) or 2,6,10,14-tetramethylpentadecane (pristane) on gene expression and transformation were examined using two clones (P+, TPA transformation sensitive and P-, TPA resistant) of the mouse epidermal cell line JB6. Results from transformation studies indicated pristane was more efficient, i.e., lower concentrations were required to elicit an equivalent response, in transforming the P+, but not the P-, clone of JB6 compared to TPA. Furthermore, results from these studies demonstrated either TPA or pristane was effective in the transactivation of the chloramphenicol acetyltransferase gene under the regulatory control of most viral promoter/enhancer elements transfected into the P+, but not the P-, clone of JB6. However, if a consensus cAMP response element was linked to the simian virus 40 early promoter, pristane activation was observed in both P+ and P- cells. The differential effects of these two compounds suggest that while they have similar characteristics, they may utilize different pathways to elicit their effects.
使用小鼠表皮细胞系JB6的两个克隆（P +，对佛波酯-13-乙酸酯[TPA]转化敏感；P -，对TPA有抗性）研究了12 - O - 十四烷酰佛波醇-13-乙酸酯（TPA）或2,6,10,14-四甲基十五烷（降植烷）对基因表达和转化的影响。转化研究结果表明，与TPA相比，降植烷在转化JB6的P +克隆（而非P -克隆）方面更有效，即引发同等反应所需的浓度更低。此外，这些研究结果表明，TPA或降植烷在转染到JB6的P +克隆（而非P -克隆）中的大多数病毒启动子/增强子元件的调控下，均能有效激活氯霉素乙酰转移酶基因。然而，如果将一个共有cAMP反应元件与猿猴病毒40早期启动子相连，则在P +和P -细胞中均观察到降植烷激活。这两种化合物的不同作用表明，虽然它们具有相似的特性，但可能利用不同的途径来发挥其作用。
