Kumatori A, Tanaka K, Tamura T, Fujiwara T, Ichihara A, Tokunaga F, Onikura A, Iwanaga S
Institute for Enzyme Research, University of Tokushima, Japan.
FEBS Lett. 1990 May 21;264(2):279-82. doi: 10.1016/0014-5793(90)80267-m.
The nucleotide sequence of component C9 of rat proteasomes (multicatalytic proteinase complexes) has been determined from a recombinant cDNA clone isolated by screening a Reuber H4TG hepatoma cell cDNA library using synthetic oligodeoxynucleotide probes corresponding to partial amino acid sequences of the protein. The predicted sequence of C9 consists of 261 amino acid residues with a calculated molecular weight of 29,496. The C9 component is a novel protein, differing from known proteins, but its primary structure resembles those of other proteasome components, including C2, C3 and C5, although its similarity to C5 is relatively low, suggesting that proteasomes consist of a family of proteins that have evolved from a common ancestor.
大鼠蛋白酶体（多催化蛋白酶复合物）的C9组分的核苷酸序列，是通过用与该蛋白质部分氨基酸序列对应的合成寡聚脱氧核苷酸探针筛选Reuber H4TG肝癌细胞cDNA文库分离出的重组cDNA克隆来确定的。C9的预测序列由261个氨基酸残基组成，计算分子量为29496。C9组分是一种新型蛋白质，与已知蛋白质不同，但其一级结构类似于其他蛋白酶体组分，包括C2、C3和C5，尽管它与C5的相似性相对较低，这表明蛋白酶体由一个从共同祖先进化而来的蛋白质家族组成。
