Aki M, Tamura T, Tokunaga F, Iwanaga S, Kawamura Y, Shimbara N, Kagawa S, Tanaka K, Ichihara A
Department of Urology, School of Medicine, University of Tokushima, Japan.
FEBS Lett. 1992 Apr 13;301(1):65-8. doi: 10.1016/0014-5793(92)80211-x.
The nucleotide sequence of a cDNA that encodes a new subunit, named RCl, of rat proteasomes (multicatalytic proteinase complexes) has been determined. The polypeptide predicted from the open reading frame consisted of 208 amino acid residues with a calculated molecular mass of 23, 130, which is consistent with the size obtained by electrophoretic analysis of purified RCl. The partial amino acid sequences of several fragments of RCl, obtained by protein chemical analyses, were found to be in excellent accordance with those deduced from the cDNA sequence. Surprisingly, the overall structure of RCl was found to be almost identical to that of recently isolated RING10, whose gene is located in the class II region of the human MHC gene cluster. This finding suggests that RCl is a homologue of human RING10, supporting the proposal that proteasomes are involved in the antigen processing pathway.
已确定了一种编码大鼠蛋白酶体（多催化蛋白酶复合物）新亚基RCl的cDNA的核苷酸序列。从开放阅读框预测的多肽由208个氨基酸残基组成，计算分子量为23130，这与通过纯化的RCl进行电泳分析得到的大小一致。通过蛋白质化学分析获得的RCl几个片段的部分氨基酸序列，与从cDNA序列推导的序列高度一致。令人惊讶的是，发现RCl的整体结构与最近分离的RING10几乎相同，其基因位于人类MHC基因簇的II类区域。这一发现表明RCl是人类RING10的同源物，支持了蛋白酶体参与抗原加工途径的提议。
