Fujita Y
First Department of Orthodontics, Faculty of Dentistry, Tokyo Medical and Dental University.
Kokubyo Gakkai Zasshi. 1993 Mar;60(1):183-98. doi: 10.5357/koubyou.60.183.
The purpose of this study was to investigate the osteopontin mRNA expression in the alveolar bone incident to the physiologic and experimental tooth movement. The upper jaws of female rats (7 weeks old) which were submitted to experimental molar tooth movement and control specimens were decalcified and sliced into paraffin sections. Osteopontin mRNA expression and tartrate-resistant acid phosphatase (TRACPase) activity were examined in the alveolar bone by use of in situ hybridization techniques and enzyme histochemistry, respectively. In the control specimens high levels of osteopontin mRNA expression was observed in the osteocytes and lining cells close to the osteoclasts which were detected by TRACPase staining in the distal areas of the tooth sockets. In the mesial tooth socket areas however, low levels of osteopontin mRNA expression was observed. In the experimental specimens high levels of osteopontin mRNA expression of both the osteoblasts and osteocytes was observed in the mesial areas of tooth sockets in response to the experimental tooth movement. These results indicated that osteopontin gene expression is related to the alveolar bone remodeling as far as the bone resorption incident to the physiologic tooth movement and that this expression level significantly increased in response to a certain mechanical stress incident to the experimental tooth movement.
本研究的目的是调查在生理性和实验性牙齿移动过程中，牙槽骨中骨桥蛋白mRNA的表达情况。将接受实验性磨牙移动的7周龄雌性大鼠的上颌骨和对照标本进行脱钙处理，并切成石蜡切片。分别使用原位杂交技术和酶组织化学方法检测牙槽骨中骨桥蛋白mRNA的表达和抗酒石酸酸性磷酸酶（TRACPase）的活性。在对照标本中，在牙槽远端区域通过TRACPase染色检测到的靠近破骨细胞的骨细胞和衬里细胞中观察到高水平的骨桥蛋白mRNA表达。然而，在近中牙槽区域，观察到骨桥蛋白mRNA表达水平较低。在实验标本中，响应实验性牙齿移动，在牙槽近中区域观察到成骨细胞和骨细胞中骨桥蛋白mRNA均有高水平表达。这些结果表明，就生理性牙齿移动过程中的骨吸收而言，骨桥蛋白基因表达与牙槽骨重塑有关，并且该表达水平在响应实验性牙齿移动所产生的一定机械应力时显著增加。
